An enzyme-linked immunoassay of testosterone

An enzyme-linked immunoassay of testosterone is described. The conjugation of testosterone with high specific activity horseradish peroxidase and a highly sensitive assay for this enzyme having previously been studied, here we describe the immobilization of the anti-testosterone antibody and the dev...

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Published inSteroids Vol. 30; no. 1; pp. 129 - 137
Main Authors Rajkowski, K.M., Cittanova, N., Urios, P., Jayle, M.F.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.07.1977
Subjects
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Estradiol - immunology
Gonadal Steroid Hormones - immunology
Radioimmunoassay
Testosterone - analysis
Testosterone - immunology
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Abstract An enzyme-linked immunoassay of testosterone is described. The conjugation of testosterone with high specific activity horseradish peroxidase and a highly sensitive assay for this enzyme having previously been studied, here we describe the immobilization of the anti-testosterone antibody and the development of assay conditions permitting the determination of 50 pg to 1.5 ng testosterone in one working day. In this work attention has been paid to keeping the assay method as simple as possible. The method is discussed in terms of other enzyme-linked immunoassays for steroids.
AbstractList An enzyme-linked immunoassay of testosterone is described. The conjugation of testosterone with high specific activity horseradish per-oxidase and a highly sensitive assay for this enzyme having previously been studied, here we describe the immobilization of the anti-testosterone antibody and the development of assay conditions permitting the determination of 50 pg to 1.5 ng testosterone in one working day. In this work attention has been paid to keeping the assay method as simple as possible. The method is discussed in terms of other enzyme-linked immunoassays for steroids.
An enzyme-linked immunoassay of testosterone is described. The conjugation of testosterone with high specific activity horseradish peroxidase and a highly sensitive assay for this enzyme having previously been studied, here we describe the immobilization of the anti-testosterone antibody and the development of assay conditions permitting the determination of 50 pg to 1.5 ng testosterone in one working day. In this work attention has been paid to keeping the assay method as simple as possible. The method is discussed in terms of other enzyme-linked immunoassays for steroids.
Author Jayle, M.F.
Cittanova, N.
Urios, P.
Rajkowski, K.M.
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Cites_doi 10.1016/0014-5793(72)80830-5
10.1038/2141302a0
10.1016/0005-2795(71)90132-2
10.1007/BF00440812
10.1016/0039-128X(73)90050-0
10.1016/0005-2744(75)90016-9
10.1016/S0300-9084(72)80004-X
10.1530/acta.0.062S207
10.1016/S0021-9258(18)98136-6
10.1016/0022-1759(76)90167-8
10.1016/0014-5793(74)80456-4
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Engvall, Jonsson, Perlman (BIB6) 1971; 251
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Dray, Andrieu, Renaud (BIB5) 1975; 403
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Van Weeman, Schuurs (BIB2) 1971; 15
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Erlanger (10.1016/0039-128X(77)90143-X_BIB11) 1959; 234
Kleinhammer (10.1016/0039-128X(77)90143-X_BIB7) 1976; 279
De Lauzon (10.1016/0039-128X(77)90143-X_BIB10) 1973; 22
Avrameas (10.1016/0039-128X(77)90143-X_BIB4) 1972; 54
Kato (10.1016/0039-128X(77)90143-X_BIB9) 1975; 78
Van Weeman (10.1016/0039-128X(77)90143-X_BIB3) 1972; 24
Comoglio (10.1016/0039-128X(77)90143-X_BIB14) 1976; 10
Axen (10.1016/0039-128X(77)90143-X_BIB12) 1967; 214
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Snippet An enzyme-linked immunoassay of testosterone is described. The conjugation of testosterone with high specific activity horseradish peroxidase and a highly...
An enzyme-linked immunoassay of testosterone is described. The conjugation of testosterone with high specific activity horseradish per-oxidase and a highly...
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SubjectTerms Cross Reactions
Enzyme-Linked Immunosorbent Assay
Estradiol - immunology
Gonadal Steroid Hormones - immunology
Radioimmunoassay
Testosterone - analysis
Testosterone - immunology
Title An enzyme-linked immunoassay of testosterone
URI https://dx.doi.org/10.1016/0039-128X(77)90143-X
https://www.ncbi.nlm.nih.gov/pubmed/919013
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