Low sodium isocyanurate concentrations as a substitute to medium autoclaving in plant tissue culture
An optimization for a medium sterilization method, capable of substituting autoclaving, was developed using low concentrations of sodium isocyanurate (ISO) as a medium sterilizer. Sodium isocyanurate was used in it’s dichloro form (sodium dichloroisocyanurate) with concentrations on medium ranging f...
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Published in | Plant cell, tissue and organ culture Vol. 139; no. 3; pp. 601 - 604 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.12.2019
Springer Nature B.V |
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Abstract | An optimization for a medium sterilization method, capable of substituting autoclaving, was developed using low concentrations of sodium isocyanurate (ISO) as a medium sterilizer. Sodium isocyanurate was used in it’s dichloro form (sodium dichloroisocyanurate) with concentrations on medium ranging from 0.04 to 0.005 g/L. The chosen specimen was
Dianthus caryophyllus
, placed in Murashige and Skoog media as seeds. Sterilization using sodium isocyanurate surpassed that of medium autoclaving on time spent, resources required and sterilization capability. All successful ISO concentrations, 0.04 g/L to 0.01 g/L, allowed
D. caryophyllus
sprouts to develop with no signs of phytotoxic effects and with constant growth until they reached their container’s maximum capacity. Contamination rates for concentrations 0.04 g/L, 0.02 and 0.01 g/L stayed bellow 5% for the entirety of the experiment. The data presented, along with how this work was conducted, indicate that sodium isocyanurate is capable of substituting media autoclaving for practices with
D. caryophyllus
seeds and opens a path for further research involving sodium isocyanurate sterilization for other species.
Key message
This protocol is an optimization for an already existing medium sterilization protocol, in plant tissue culture, through chemical compounds. Sodium isocyanurate was capable of sterilizing culture media in varied concentrations while showing no signs of phytotoxicity to
Dianthus caryophyllus
seeds and subsequent sprouts. |
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AbstractList | An optimization for a medium sterilization method, capable of substituting autoclaving, was developed using low concentrations of sodium isocyanurate (ISO) as a medium sterilizer. Sodium isocyanurate was used in it’s dichloro form (sodium dichloroisocyanurate) with concentrations on medium ranging from 0.04 to 0.005 g/L. The chosen specimen was Dianthus caryophyllus, placed in Murashige and Skoog media as seeds. Sterilization using sodium isocyanurate surpassed that of medium autoclaving on time spent, resources required and sterilization capability. All successful ISO concentrations, 0.04 g/L to 0.01 g/L, allowed D. caryophyllus sprouts to develop with no signs of phytotoxic effects and with constant growth until they reached their container’s maximum capacity. Contamination rates for concentrations 0.04 g/L, 0.02 and 0.01 g/L stayed bellow 5% for the entirety of the experiment. The data presented, along with how this work was conducted, indicate that sodium isocyanurate is capable of substituting media autoclaving for practices with D. caryophyllus seeds and opens a path for further research involving sodium isocyanurate sterilization for other species. An optimization for a medium sterilization method, capable of substituting autoclaving, was developed using low concentrations of sodium isocyanurate (ISO) as a medium sterilizer. Sodium isocyanurate was used in it’s dichloro form (sodium dichloroisocyanurate) with concentrations on medium ranging from 0.04 to 0.005 g/L. The chosen specimen was Dianthus caryophyllus , placed in Murashige and Skoog media as seeds. Sterilization using sodium isocyanurate surpassed that of medium autoclaving on time spent, resources required and sterilization capability. All successful ISO concentrations, 0.04 g/L to 0.01 g/L, allowed D. caryophyllus sprouts to develop with no signs of phytotoxic effects and with constant growth until they reached their container’s maximum capacity. Contamination rates for concentrations 0.04 g/L, 0.02 and 0.01 g/L stayed bellow 5% for the entirety of the experiment. The data presented, along with how this work was conducted, indicate that sodium isocyanurate is capable of substituting media autoclaving for practices with D. caryophyllus seeds and opens a path for further research involving sodium isocyanurate sterilization for other species. Key message This protocol is an optimization for an already existing medium sterilization protocol, in plant tissue culture, through chemical compounds. Sodium isocyanurate was capable of sterilizing culture media in varied concentrations while showing no signs of phytotoxicity to Dianthus caryophyllus seeds and subsequent sprouts. |
Author | Araujo Figueiredo, Sergio da Costa Urtiga, Caio de Araújo Silva-Cardoso, Inaê Mariê |
Author_xml | – sequence: 1 givenname: Caio orcidid: 0000-0002-1366-2519 surname: da Costa Urtiga fullname: da Costa Urtiga, Caio organization: Department of Botany, University of Brasília – sequence: 2 givenname: Inaê Mariê surname: de Araújo Silva-Cardoso fullname: de Araújo Silva-Cardoso, Inaê Mariê organization: Department of Forest Engineering, University of Brasília – sequence: 3 givenname: Sergio surname: Araujo Figueiredo fullname: Araujo Figueiredo, Sergio email: sergioaf@unb.br organization: Department of Botany, University of Brasília |
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Cites_doi | 10.1111/j.1399-3054.1962.tb08052.x 10.1007/978-1-4939-8594-4 10.1007/s11240-006-9121-3 10.1007/s11627-011-9418-8 10.1007/s00299-004-0837-4 10.1007/s12355-012-0178-5 10.9790/30210-0703016165 10.1007/s12210-017-0623-1 10.1002/14356007.a08_191 10.5902/198050982749 10.17660/ActaHortic.2007.764.40 |
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Keywords | Medium autoclaving Chlorine disinfectant Plant tissue culture Medium contamination Chemical sterilization |
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References | Cardoso, Teixeira da Silva (CR2) 2012; 48 Cardoso, Gerald, Silva, Loyola-Vargas, Ochoa-Alejo (CR3) 2018 Ribeiro, Teixeira, Bastos (CR10) 2011; 21 Tiwari, Tripathi, Lal, Mishra (CR12) 2012; 14 CR6 Teixeira, Ribeiro, Teixeira (CR11) 2006; 86 (CR9) 2015 Murashige, Skoog (CR8) 1962; 15 Weathers, DeJesus-Gonzalez, Kim, Souret, Towler (CR13) 2004; 23 Huthmacher, Most (CR4) 2000 Macêdo (CR7) 2017; 10 Al-Snafi (CR1) 2017; 7 Lukatkin, Mokshin, Teixeira da Silva (CR5) 2017; 28 Yanagawa, Tanaka, Funai (CR14) 2007; 764 JM Ribeiro (1681_CR10) 2011; 21 T Yanagawa (1681_CR14) 2007; 764 T Murashige (1681_CR8) 1962; 15 R CORE TEAM (1681_CR9) 2015 SL Teixeira (1681_CR11) 2006; 86 K Huthmacher (1681_CR4) 2000 JC Cardoso (1681_CR3) 2018 AE Al-Snafi (1681_CR1) 2017; 7 JAB Macêdo (1681_CR7) 2017; 10 JC Cardoso (1681_CR2) 2012; 48 AS Lukatkin (1681_CR5) 2017; 28 AK Tiwari (1681_CR12) 2012; 14 PJ Weathers (1681_CR13) 2004; 23 1681_CR6 |
References_xml | – volume: 15 start-page: 473 year: 1962 end-page: 497 ident: CR8 article-title: A revised medium for rapid growth and bio assays with tobacco tissue cultures publication-title: Physiol Plant doi: 10.1111/j.1399-3054.1962.tb08052.x contributor: fullname: Skoog – start-page: 17 year: 2018 end-page: 46 ident: CR3 article-title: Micropropagation in the twenty-first century publication-title: Plant cell culture protocols. Methods in molecular biology doi: 10.1007/978-1-4939-8594-4 contributor: fullname: Ochoa-Alejo – volume: 86 start-page: 375 year: 2006 end-page: 378 ident: CR11 article-title: Influence of NaOCl on nutrient medium sterilization and on pineapple ( cv. Smooth cayenne) behavior publication-title: Plant Cell Tissue Org Cult doi: 10.1007/s11240-006-9121-3 contributor: fullname: Teixeira – year: 2015 ident: CR9 publication-title: R: a language and environment for statistical computing – volume: 48 start-page: 362 year: 2012 end-page: 368 ident: CR2 article-title: Micropropagation of gerbera using chlorine dioxide (ClO ) to sterilize the culture medium publication-title: In Vitro Cell Dev Biol Plant doi: 10.1007/s11627-011-9418-8 contributor: fullname: Teixeira da Silva – volume: 23 start-page: 414 year: 2004 end-page: 418 ident: CR13 article-title: Alteration of biomass and artemisinin production in hairy roots by media sterilization method and sugars publication-title: Plant Cell Rep doi: 10.1007/s00299-004-0837-4 contributor: fullname: Towler – volume: 14 start-page: 364 issue: 4 year: 2012 end-page: 369 ident: CR12 article-title: Screening of some chemical disinfectants for media sterilization during in vitro micropropagation of sugarcane publication-title: Sugar Tech doi: 10.1007/s12355-012-0178-5 contributor: fullname: Mishra – ident: CR6 – volume: 7 start-page: 61 issue: 3 year: 2017 end-page: 71 ident: CR1 article-title: Chemical contents and medical importance of —a review publication-title: IOSR J Pharm doi: 10.9790/30210-0703016165 contributor: fullname: Al-Snafi – volume: 28 start-page: 583 year: 2017 end-page: 588 ident: CR5 article-title: Use of alternative plant growth regulators and carbon sources to manipulate L. shoot induction in vitro publication-title: Rend Fis Acc Lincei doi: 10.1007/s12210-017-0623-1 contributor: fullname: Teixeira da Silva – year: 2000 ident: CR4 article-title: Cyanuric acid and cyanuric chloride publication-title: Ullmann’s Encycl Ind Chem doi: 10.1002/14356007.a08_191 contributor: fullname: Most – volume: 10 start-page: 20 issue: 02 year: 2017 end-page: 45 ident: CR7 article-title: State of the art: sodium dichloroisocyanurate tablets X sodium dichloroisocyanurate effervescent tablets for disinfecting water in trucks tanks publication-title: Revinter contributor: fullname: Macêdo – volume: 21 start-page: 77 year: 2011 end-page: 82 ident: CR10 article-title: In vitro culture of L. on nutritive media sterilized with sodium hypochlorite publication-title: Ciência Florestal doi: 10.5902/198050982749 contributor: fullname: Bastos – volume: 764 start-page: 289 year: 2007 end-page: 298 ident: CR14 article-title: Simple micropropagation of ornamentals by direct application of chlorine disinfectants without equipment publication-title: Acta Hortic ISHS doi: 10.17660/ActaHortic.2007.764.40 contributor: fullname: Funai – volume: 48 start-page: 362 year: 2012 ident: 1681_CR2 publication-title: In Vitro Cell Dev Biol Plant doi: 10.1007/s11627-011-9418-8 contributor: fullname: JC Cardoso – volume: 86 start-page: 375 year: 2006 ident: 1681_CR11 publication-title: Plant Cell Tissue Org Cult doi: 10.1007/s11240-006-9121-3 contributor: fullname: SL Teixeira – volume: 21 start-page: 77 year: 2011 ident: 1681_CR10 publication-title: Ciência Florestal doi: 10.5902/198050982749 contributor: fullname: JM Ribeiro – volume-title: R: a language and environment for statistical computing year: 2015 ident: 1681_CR9 contributor: fullname: R CORE TEAM – ident: 1681_CR6 – volume: 14 start-page: 364 issue: 4 year: 2012 ident: 1681_CR12 publication-title: Sugar Tech doi: 10.1007/s12355-012-0178-5 contributor: fullname: AK Tiwari – volume: 764 start-page: 289 year: 2007 ident: 1681_CR14 publication-title: Acta Hortic ISHS doi: 10.17660/ActaHortic.2007.764.40 contributor: fullname: T Yanagawa – volume: 23 start-page: 414 year: 2004 ident: 1681_CR13 publication-title: Plant Cell Rep doi: 10.1007/s00299-004-0837-4 contributor: fullname: PJ Weathers – volume: 10 start-page: 20 issue: 02 year: 2017 ident: 1681_CR7 publication-title: Revinter contributor: fullname: JAB Macêdo – year: 2000 ident: 1681_CR4 publication-title: Ullmann’s Encycl Ind Chem doi: 10.1002/14356007.a08_191 contributor: fullname: K Huthmacher – volume: 28 start-page: 583 year: 2017 ident: 1681_CR5 publication-title: Rend Fis Acc Lincei doi: 10.1007/s12210-017-0623-1 contributor: fullname: AS Lukatkin – volume: 15 start-page: 473 year: 1962 ident: 1681_CR8 publication-title: Physiol Plant doi: 10.1111/j.1399-3054.1962.tb08052.x contributor: fullname: T Murashige – start-page: 17 volume-title: Plant cell culture protocols. 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SubjectTerms | Autoclaving Biomedical and Life Sciences Chemical compounds Contamination Culture media Dianthus caryophyllus Life Sciences Low concentrations Optimization Organic chemistry Phytotoxicity Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Plant tissues Research Note Seeds Sodium Sodium dichloroisocyanurate Sterilization Tissue culture |
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Title | Low sodium isocyanurate concentrations as a substitute to medium autoclaving in plant tissue culture |
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