Transcription of the rpsO-pnp operon of Streptomyces coelicolor involves four temporally regulated, stress responsive promoters

• Published in: Gene Vol. 536; no. 1; pp. 177 - 185
• Main Authors: Bralley, Patricia, Gatewood, Marcha L., Jones, George H.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.02.2014
• Subjects: Bacterial Proteins - genetics; Base Sequence; Cold shock; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Operon - genetics; Polynucleotide phosphorylase; Polyribonucleotide Nucleotidyltransferase - genetics; Promoter; Promoter Regions, Genetic; Ribonuclease III - metabolism; Ribosomal Proteins - genetics; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; rpsO-pnp; Sigma factor; Streptomyces; Streptomyces coelicolor - genetics; Stress, Physiological - genetics; Transcription, Genetic - genetics; Promoter; SMM; rpsO-pnp; PNPase; Cold shock; APE; CATO2ase; Streptomyces; Sigma factor; SFM; Polynucleotide phosphorylase; PCR; catechol dioxygenase; CATOase; acetone-phosphate-EDTA; soya flour-mannitol; Streptomyces minimal medium; polymerase chain reaction

Primer extension with RNA from an RNase III null mutant of Streptomyces coelicolor M145 and a primer complementary to the polynucleotide phosphorylase gene revealed two major extension products. Two different extension products were observed using RNA from either wild type M145 or the null mutant wi...