Reactive oxygen species activated NLRP3 inflammasomes initiate inflammation in hyperosmolarity stressed human corneal epithelial cells and environment-induced dry eye patients

• Published in: Experimental eye research Vol. 134; pp. 133 - 140
• Main Authors: Zheng, Qinxiang, Ren, Yueping, Reinach, Peter S., Xiao, Bing, Lu, Huihui, Zhu, Yirui, Qu, Jia, Chen, Wei
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2015
• Subjects: Acetylcysteine - pharmacology; Adult; Blotting, Western; Carrier Proteins - metabolism; Caspase 1 - metabolism; Caspase-1; Cell Line; Dry eye; Dry Eye Syndromes - metabolism; Enzyme-Linked Immunosorbent Assay; Epithelium, Corneal - metabolism; Female; Gene Knockdown Techniques; Humans; IL-1β; Inflammasomes; Inflammasomes - metabolism; Inflammation; Inflammation - metabolism; Interleukin-1beta - metabolism; Keratitis - metabolism; Male; Middle Aged; NLR Family, Pyrin Domain-Containing 3 Protein; NLRP3; Osmotic Pressure - drug effects; Reactive oxygen species; Reactive Oxygen Species - metabolism; Real-Time Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Small Interfering - genetics; Signal Transduction; Tear film hyperosmolarity; Transfection; Young Adult; APCs; DE; Reactive oxygen species; Inflammasomes; Inflammation; IL-1β; OSDI; Caspase-1; ASC; MMP; NAC; PRR; Dry eye; NLR; ROS; HCEC; NLRP3; Tear film hyperosmolarity; TLR

In studies on dry eye (DE) disease, an association has been identified between tear film hyperosmolarity and inflammation severity elicited through receptor-induced increases in proinflammatory cytokine and chemokine release. These immune reactions might be mediated by inflammasomes, macromolecular...