Distribution of GSTM1, GSTT1, GSTP1 and TP53 disease-associated gene variants in native and urban Venezuelan populations

• Published in: Gene Vol. 531; no. 1; pp. 106 - 111
• Main Authors: Chiurillo, Miguel Angel, Griman, Pedro, Santiago, Laskhmi, Torres, Keila, Moran, Yeinmy, Borjas, Lisbeth
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2013
• Subjects: Alleles; Amerindians; Gene Frequency; Genetic Variation; Genotype; Glutathione S-Transferase pi - genetics; Glutathione Transferase - genetics; GSTM1; GSTP1; GSTT1; Humans; Mutation; Neoplasms - genetics; TP53; Tumor Suppressor Protein p53 - genetics; Venezuela - ethnology; Venezuelan population; Venezuela; GSTM1; GSTP1; GSTs; GSTT1; Venezuelan population; Amerindians; SNP; DNA; HWE; LD; TP53; PCR; RFLP; glutathione S-transferase T1; glutathione S-transferase P1; glutathione S-transferase M1; restriction fragment length polymorphism; Hardy–Weinberg equilibrium; deoxyribonucleic acid; single-nucleotide polymorphism; polymerase chain reaction; glutathione S-transferases; linkage disequilibrium
• DOI: 10.1016/j.gene.2013.08.055
• ISSN: 0378-1119

The contemporary Venezuelan population is the product of major admixture process across various historical events, which has provided it a particular genetic background. The aim of this study concerns the analysis of glutathione S-transferase (GST) GSTM1, GSTP1 and GSTT1 genetic variants and five polymorphisms at the TP53 gene, which are related to cancer susceptibility, in an urban/admixed population and five Amerindian tribes (Bari, Panare, Pemon, Warao and Wayuu) from Venezuela. Genotyping was carried out in 120 individuals from an urban sample and 188 Amerindians. The analysis performed on TP53 haplotype and GST allele distribution showed a close correlation for Pemon and Warao populations, while Bari group appears isolated from the other populations. GSTT1 null variant frequency in our admixed (11%) and native samples (0.0–11.4%) was lower when compared with Caucasians, Africans and Asians. Frequency of the GSTP1*Val cancer-associated allele found in Bari (88.6%) and Panare (63.0%) is of the highest so far reported. Fourteen TP53 haplotypes were observed in the admixed populations, whereas only 3 to 5 in Amerindians. To our knowledge this is the first report of GST polymorphisms and TP53 haplotype distribution in Venezuelans. The distribution of most of analyzed polymorphisms in the urban sample is consistent with the admixed origin of the present-day population of Venezuela. While, the inter-ethnic variations in genetic polymorphisms found in Native American tribes seem to be the result of the influence of demographic factors. These results provide additional data for undertaking ethnographic and disease association studies in Venezuela. •First report of the distribution of GST and TP53 variants in Venezuelan populations.•The data may provide a basic database for the future clinical and genetic studies.•Results are consistent with the admixed origin of the population of Venezuela.•Frequency of the GSTP1*Val risk-associated allele are of the highest so far reported.•SMTNL2 is highly expressed in skeletal muscle and several other tissues.