Localization of tissue transglutaminase and N (epsilon)-(gamma) -glutamyl lysine in duodenal cucosa during the development of mucosal atrophy in coeliac disease

• Published in: Virchows Archiv : an international journal of pathology Vol. 446; no. 6; pp. 613 - 618
• Main Authors: Sakly, Wahiba, Sriha, Badreddine, Ghedira, Ibtissem, Bienvenu, Françoise, Ayadi, Abdelkarim, Tahar Sfar, Mohamed, Lachaux, Alain, Korbi, Sadok, Bienvenu, Jacques, Fabien, Nicole
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.06.2005; Springer Nature B.V
• Subjects: Adolescent; Adult; Atrophy - metabolism; Atrophy - pathology; Biological and medical sciences; Celiac disease; Celiac Disease - metabolism; Celiac Disease - pathology; Child; Child, Preschool; Dipeptides - metabolism; Duodenum - metabolism; Duodenum - pathology; Female; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Immunohistochemistry; Infant; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Investigative techniques, diagnostic techniques (general aspects); Male; Medical sciences; Middle Aged; Other diseases. Semiology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Transglutaminases - metabolism; Duodenum; Digestive system; Enzyme; Transferases; Mucosa; Coeliac disease; Aminoacyltransferases; Small intestine; Atrophy; Duodenal mucosal atrophy; Tissue; Anatomic pathology; Intestinal malabsorption; Lysine; Development; Tissue transglutaminase; Digestive diseases; Intestinal disease; Protein-glutamine γ-glutamyltransferase; Localization; Nε-(γ-glutamyl) lysine isopeptide
• ISSN: 0945-6317; 1432-2307
• DOI: 10.1007/s00428-005-1237-z

Expression and transamidation activity of tissue transglutaminase (tTG) may be involved in the morphological modifications leading to the mucosal atrophy observed in coeliac disease (CD). We aimed to investigate the localization of tTG within the duodenal mucosa during the development of villous atrophy. The localization and level of expression of N epsilon-(gamma-glutamyl) lysine isopeptides which could reflect the transamidation activity of tTG were also analyzed. tTG and N epsilon-(gamma-glutamyl) lysine were localized using an immunohistochemical technique on duodenal biopsies obtained from 75 patients with CD and 51 subjects with normal mucosa (control group). The number of cases displaying tTG-expressing cells in the basement membrane and lamina propria was significantly higher in CD patients than in the control group. Moreover, the intensity of tTG staining in these areas was higher in CD. In contrast, the number of biopsies with tTG-expressing enterocytes was significantly lower in CD than in the control group. There was no difference in N epsilon-(gamma-glutamyl) lysine between the two populations. Tissue transglutaminase was differently expressed in the various areas of the mucosa according to the stage of atrophy, whereas the localization and the intensity of the labelling of N epsilon-(gamma-glutamyl) lysine isopeptides did not show any modification. The preferential localization in the basement membrane and lamina propria may reflect the involvement of tTG in the development of mucosal atrophy in CD.