Effect of single-use versus combined-use moschus and diazepam on expression of amino acid neurotransmitters in the rat corpus striatum

• Published in: Neural regeneration research Vol. 7; no. 3; pp. 182 - 186
• Main Authors: Zhang, Na, Liu, Ping, He, Xinrong
• Format: Journal Article
• Language: English
• Published: India Medknow Publications & Media Pvt. Ltd 25.01.2012; Traditional Chinese Medicine Pharmacy, Department of Pharmaceutical Care, Chinese PLA General Hospital, Beijing 100853, China; Department of Pharmacy, Shijiazhuang Fourth Hospital, Shijiazhuang 050011, Hebei Province, China%Traditional Chinese Medicine Pharmacy, Department of Pharmaceutical Care, Chinese PLA General Hospital, Beijing 100853, China; Medknow Publications & Media Pvt Ltd
• Subjects: Amino acids; Chromatography; Drug dosages; Hemodialysis; Laboratory animals; Medical research; Nervous system; Neurotransmitters; Pharmaceuticals; Pharmacy; Research and Report: Traditional Chinese Medicine and Neuroregeneration; Traditional Chinese medicine; 兴奋性神经递质; 反相高效液相色谱法; 地西泮; 天门冬氨酸; 抑制性神经递质; 氨基酸类神经递质; 联合使用; 麝香鼠; Beijing China; United States > US; China; moschus; microdialysis; diazepam; glycine; γ-aminobutyric acid; high-performance liquid chromatography; aspartate; neurotransmitter; glutamate
• ISSN: 1673-5374; 1876-7958
• DOI: 10.3969/j.issn.1673-5374.2012.03.004

The present study analyzed expressional changes of excitatory neurotransmitters and inhibitory neurotransmitters in the rat corpus striatum after single-use and combined-use diazepam and Chinese herb moschus. The influence of moschus on the central nervous system was analyzed, in particular whether moschus increased penetration of other drugs into the brain. Reverse-phase high-performance liquid chromatography, which included pre-column derivation with orthophthaladehyde detection, showed varied increased levels of excitatory neurotransmitters, including aspartate and glutamate, and inhibitory neurotransmitters, including glycine and Y-aminobutyric acid, in the corpus striatum after treatment with moschus alone, diazepam alone, or a combination of both. Compared with the diazepam group, aspartate levels significantly decreased at 30 and 60-105 minutes after combined treatment with moschus, while glutamate significantly increased at 45 and 75-105 minutes, glycine levels significantly increased at 105 minutes, and γ-aminobutyric acid increased at 30 and 75-105 minutes. These findings suggested that moschus increased the inhibition effects of diazepam on the brain.