Orf239342 from the mushroom Agaricus bisporus is a mannose binding protein

• Published in: Biochemical and biophysical research communications Vol. 515; no. 1; pp. 99 - 103
• Main Authors: Rachmawati, Heni, Sundari, Syaripah, Nabila, Najwa, Tandrasasmita, Olivia M., Amalia, Riezki, Siahaan, Teruna J., Tjandrawinata, Raymond R., Ismaya, Wangsa T.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.07.2019
• Subjects: Light subunit of mushroom tyrosinase; Mannose-binding protein; Mushroom tyrosinase associated lectin-like protein; Orf239342; Mushroom tyrosinase associated lectin-like protein; Mannose-binding protein; Light subunit of mushroom tyrosinase; Orf239342
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2019.05.107

A recently discovered lectin-like protein from mushroom tyrosinase designated as orf239342 inhibits proliferation of the MCF-7 breast cancer cells. This characteristic is likely derived from its ability to recognize sugar entity on the cell surface. Thereby, the binding specificity of orf239342 to sugars was studied. Orf239342 was found to bind specifically to mannose upon analysis with the surface plasmon resonance technique. Finally, our in vitro study showed that mannose impeded orf239342 ability to inhibit proliferation of the MCF-7 breast cancer cells, providing further evidence for the mannose binding onto the protein. Our finding is a breakthrough to characterise orf239342 i.e. to define its functioning in the mushroom, association to the tyrosinase, or even possible application in breast cancer therapy. In addition, the finding allows the more appropriate designation of the protein as Agaricus bisporus mannose binding-protein (AbMb). •Orf239342 is a mannose binding protein.•Orf239342 inhibits proliferation of breast cancer cells.•Mannose impedes Orf239342 anti-proliferative activity.