Demonstration of beta-tropomyosin (Tpm2) and duplication of the alpha-slow tropomyosin gene (TPM3) in Atlantic salmon Salmo salar

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 245; p. 110439
• Main Authors: Silva, A. Madhushika M., Kennedy, Luke S., Hasan, Stephanie C., Cohen, Alejandro M., Heeley, David H.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.07.2020
• Subjects: Amino Acid Sequence - genetics; Animal Fins - metabolism; Animals; Atlantic salmon; Beta tropomyosin; Cheek; Electrophoresis, Gel, Two-Dimensional; Gene duplication; Genes, Duplicate; Isoform distribution; Jaw - metabolism; Mass Spectrometry; Muscle, Skeletal - metabolism; Phylogeny; Protein Isoforms - genetics; Salmo salar - genetics; Salmo salar - metabolism; Sequence Alignment; Tongue - metabolism; Tropomyosin - chemistry; Tropomyosin - genetics; Tropomyosin - metabolism; Troponin affinity; Atlantic salmon; Gene duplication; Beta tropomyosin; Troponin affinity; Isoform distribution

Beta tropomyosin (Tpm2) is demonstrated for the first time at the protein level in a fish species, using a combination of electrophoresis, mass spectrometric peptide mapping and end-group analysis. Tpm2 accounts for 50% of the total tropomyosin in slow trunk muscle of the adult Atlantic salmon as de...