Pyrosequencing on a glass surface

• Published in: Lab on a chip Vol. 16; no. 6; pp. 1063 - 1071
• Main Authors: Almeida, Ana V, Manz, Andreas, Neužil, Pavel
• Format: Journal Article
• Language: English
• Published: England 01.01.2016
• Subjects: Coating; Deoxyribonucleic acid; DNA - genetics; Droplets; Evaporation; Glass; Glass - chemistry; Humans; Magnetic fields; Microfluidic Analytical Techniques - instrumentation; Microfluidics; Sequence Analysis, DNA - instrumentation; Sequence Analysis, DNA - methods; Stations; Surface Properties

We demonstrate the use of open-surface microfluidics to sequence DNA by pyrosequencing at the plain hydrophobically coated surface of a microscope glass cover slip. This method offers significant advantages in terms of instrument size, simplicity, disposability, and functional integration, particula...