Pepsin-solubilized collagen of human nucleus pulposus and annulus fibrosus

• Published in: Biochimica et biophysica acta Vol. 434; no. 2; pp. 390 - 405
• Main Authors: Osebold, William R., Pedrini, Vittorio
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.06.1976
• Subjects: Adolescent; Adult; Age Factors; Amino Acids - analysis; Animals; Bone and Bones; Cartilage - analysis; Cattle; Child; Collagen - isolation & purification; Hexoses - analysis; Humans; Hydroxyproline - analysis; Intervertebral Disc - analysis; Macromolecular Substances; Microscopy, Electron; Organ Specificity; Pepsin A; Protein Binding; Protein Conformation; Rats; Tendons - analysis; SDS

Human nucleus pulposus and annulus fibrosus, obtained at autopsy from patients 7–30 years of age, were extracted with 2 M guanidine HCl (pH 5.82) to remove proteoglycans, then stirred with pepsin in 0.5 M acetic acid, followed by three 24-h extractions with 1 M NaCl (pH 7.5) and one 24-h extraction...