Picture perfect: Imaging mitochondrial membrane potential changes in retina slices with minimal stray fluorescence

• Published in: Experimental eye research Vol. 202; p. 108318
• Main Authors: Haider, Syeda Zehra, Mohanraj, Nivedha, Markandeya, Yogananda S., Joshi, Preeti G., Mehta, Bhupesh
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.01.2021
• Subjects: Animals; Fluorescence; Fluorescent Dyes - metabolism; Male; Membrane Potential, Mitochondrial - physiology; Mitochondria; Mitochondria - metabolism; Mitochondrial membrane potential; Optical Imaging - methods; Organometallic Compounds - metabolism; Physiology; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species - metabolism; Retina; Retina - metabolism; Vision; Fluorescence; Retina; Mitochondria; Physiology; Vision; Mitochondrial membrane potential

Mitochondrial membrane potential (Ψm) is a critical parameter that can be used to determine cellular well-being. As it is a direct measure of the cell's ATP generating capability, in recent years, this key component in cell biology has been the subject of thousands of biochemical and biophysica...