HILIC-UPLC-MS for high throughput and isomeric N-glycan separation and characterization in Congenital Disorders Glycosylation and human diseases

• Published in: Glycoconjugate journal Vol. 38; no. 2; pp. 201 - 211
• Main Authors: Messina, Angela, Palmigiano, Angelo, Esposito, Francesca, Fiumara, Agata, Bordugo, Andrea, Barone, Rita, Sturiale, Luisa, Jaeken, Jaak, Garozzo, Domenico
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.04.2021; Springer Nature B.V
• Subjects: alpha-Glucosidases - metabolism; Biochemistry; Biomedical and Life Sciences; Blood Chemical Analysis - methods; Chromatography; Chromatography, High Pressure Liquid - methods; Congenital diseases; Congenital Disorders of Glycosylation - blood; Deglycosylation; Genetic disorders; Glycoproteins; Glycosylation; Humans; Ions; Isomerism; Isomers; Labeling; Life Sciences; Liquid chromatography; Mannosidases - deficiency; Mass Spectrometry and the Analysis of Glycoconjugates; Mass spectroscopy; Membrane Proteins - deficiency; Monoclonal antibodies; N-glycans; Original Article; Pathology; Polysaccharides; Polysaccharides - blood; Polysaccharides - chemistry; Spectrometry, Mass, Electrospray Ionization - methods; High throughput; LC-MS; Oligomannose type; glycans; glycomics; CDG; HILIC; Oligomannose type N-glycans; High throughput N-glycomics

N- glycan analyses may serve uncovering disease-associated biomarkers, as well as for profiling distinctive changes supporting diagnosis of genetic disorders of glycan biosynthesis named congenital disorders of glycosylation (CDG). Strategies based on liquid chromatography (LC) preferentially couple...