Simultaneous Genotyping of Three SNVs, rs5471, rs5472, and rs2000999 Involved in Serum Haptoglobin Levels by Fluorescent Probe‐Based Melting Curve Analysis

• Published in: Electrophoresis Vol. 45; no. 21-22; pp. 2028 - 2033
• Main Authors: Soejima, Mikiko, Koda, Yoshiro
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.11.2024
• Subjects: blood serum; DNA; East Asian People - genetics; electrophoresis; fluorescence; fluorescent dyes; Fluorescent Dyes - chemistry; Fluorescent indicators; Genotype; genotyping; Genotyping Techniques - methods; Ghana; haplotypes; haptoglobin; haptoglobins; Haptoglobins - analysis; Haptoglobins - genetics; Humans; introns; Japan; melting curve genotyping; Nucleotides; Polymorphism, Single Nucleotide; Populations; promoter regions; Proteins; Reproducibility of Results; rs2000999; rs5471; rs5472; Serum proteins; West African People - genetics; Japan; Ghana; rs2000999; melting curve genotyping; haptoglobin; rs5472; rs5471

ABSTRACT Haptoglobin (Hp) is a hemoglobin‐binding acute‐phase serum protein. Several single nucleotide variations (SNVs) within the Hp gene (HP) or Hp‐related protein gene (HPR), such as rs5471 (A > C) and rs5472 (A > G) in HP promoter region and rs2000999 (G > A) in intron 2 of HRP, are su...