PrfA activation in Listeria monocytogenes increases the sensitivity to class IIa bacteriocins despite impaired expression of the bacteriocin receptor

• Published in: Biochimica et biophysica acta. General subjects Vol. 1863; no. 8; pp. 1283 - 1291
• Main Authors: Farizano, Juan V., Masías, Emilse, Hsu, Fong-Fu, Salomón, Raúl A., Freitag, Nancy E., Hebert, Elvira María, Minahk, Carlos, Saavedra, Lucila
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2019
• Subjects: Antimicrobial activity; antimicrobial peptides; bacterial growth; Bacterial Proteins - metabolism; Bacteriocins; Bacteriocins - metabolism; Culture Media; glucose; Glucose - metabolism; lipids; Listeria; Listeria monocytogenes; Listeria monocytogenes - growth & development; Listeria monocytogenes - metabolism; mannose; mechanism of action; nisin; Peptide Termination Factors - metabolism; plasma membrane; proteomics; Receptors, Cell Surface - metabolism; saprophytes; transferases; Virulence; PBS; PA; Antimicrobial activity; TSB; Listeria; Man-PTS; Virulence; TE buffer; PG; CL; PrfA cells; Bacteriocins

The scope of the present work was to characterize the activity of class IIa bacteriocins in Listeria (L.) monocytogenes cells that constitutively express an activated form of PrfA, the virulence master regulator, since bacteriocin sensitivity was only characterized in saprophytic cells so far. The m...