Partial NMR assignments for uniformly (13C, 15N)-enriched BPTI in the solid state

• Published in: Journal of biomolecular NMR Vol. 16; no. 3; pp. 209 - 219
• Main Authors: McDermott, A, Polenova, T, Bockmann, A, Zilm, K W, Paulson, E K, Martin, R W, Montelione, G T, Paulsen, E K
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.03.2000
• Subjects: Amino acids; Aprotinin - chemistry; Binding sites; Carbon Isotopes; Models, Molecular; Nitrogen Isotopes; NMR; Nuclear magnetic resonance; Nuclear Magnetic Resonance, Biomolecular

We demonstrate that high-resolution multidimensional solid state NMR methods can be used to correlate many backbone and side chain chemical shifts for hydrated micro-crystalline U-13C,15N Basic Pancreatic Trypsin Inhibitor (BPTI), using a field strength of 800 MHz for protons, magic angle sample spi...