Biosugar Production from Gracilaria verrucosa with Sulfamic Acid Pretreatment and Subsequent Enzymatic Hydrolysis

• Published in: Biotechnology and bioprocess engineering Vol. 23; no. 3; pp. 302 - 310
• Main Authors: Park, Mi-Ra, Kim, Sung-Koo, Jeong, Gwi-Taek
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.06.2018; 한국생물공학회
• Subjects: active sites; biomass; biorefining; Biotechnology; catalysts; Chemistry; Chemistry and Materials Science; enzymatic hydrolysis; Gracilaria verrucosa; Industrial and Production Engineering; reducing sugars; Research Paper; 생물공학; sulfamic acid-catalyzed pretreatment; biosugars; combined severity; enzymatic hydrolysis
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-018-0090-2

In this study, sulfamic acid-catalyzed pretreatment and subsequent enzymatic hydrolysis was conducted to produce biosugar from the marine macro-alga Gracilaria verrucosa . Sulfamic acid has dual active sites and is a green catalyst. Optimized sulfamic acid pretreatment at 130°C with 7.5% biomass and 100 mM sulfamic acid for 90 min yielded 39.9% total reducing sugar (TRS). Subsequent enzymatic hydrolysis yielded 69.1% TRS. These results indicate the potential of sulfamic acidcatalyzed pretreatment and subsequent enzymatic hydrolysis in producing biosugars using a biorefinery process.