The Influence of Cooper and Chromium Ions on the Production of Exopolysaccharide and Polyhydroxybutyrate by Rhizobium tropici LBMP-C01

• Published in: Journal of polymers and the environment Vol. 27; no. 3; pp. 445 - 455
• Main Authors: Leonel, Tatiane Fernanda, Moretto, Cristiane, Castellane, Tereza Cristina Luque, da Costa, Paulo Inácio, Lemos, Eliana Gertrudes de Macedo
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2019; Springer Nature B.V
• Subjects: Biopolymers; Bioremediation; Chemistry; Chemistry and Materials Science; Chromium; Copper; Environmental Chemistry; Environmental Engineering/Biotechnology; Exopolysaccharides; Gene expression; Hexavalent chromium; Industrial Chemistry/Chemical Engineering; Materials Science; Metal ions; Original Paper; Polyhydroxybutyrate; Polyhydroxybutyric acid; Polymer Sciences; Polymerase chain reaction; Real time; Supplements; Rhizobia; Exopolysaccharide; Polyhydroxybutyrate; Heavy metals
• ISSN: 1566-2543; 1572-8919
• DOI: 10.1007/s10924-018-1359-4

The influence of bivalent copper (Cu 2+ ) and hexavalent chromium (Cr 6+ ) ions on exopolysaccharide (EPS) and polyhydroxybutyrate (PHB) production by the bacterium Rhizobium tropici LBMP-C01 was investigated. The partial structure of both biopolymers produced by rhizobia were characterized, and the quantification of exo R and exo Z gene expression by real-time polymerase chain reaction (Real-Time PCR) was also investigated. Results showed that the supplementation with Cu 2+ for 144 h induced an increase in the production of EPS by 48% and PHB by 46.66% compared to the control. Expression studies revealed that the expressed exo R and e xo Z genes were affected by metal ion treatment. It can be stated that this particular rhizobial strain is a future potential candidates to produce super adsorbent quite useful for soil bioremediation.