The influence of stimulated peritoneal feeder cells and mitogens upon antibody secreting hybridomas

• Published in: Hybridoma Vol. 7; no. 3; p. 289
• Main Authors: Lane, R D, Renno, W, Nepomuceno, V, Schafer, C, Mellgren, R L
• Format: Journal Article
• Language: English
• Published: United States 01.06.1988
• Subjects: Animals; Antibody-Producing Cells - drug effects; Hybridomas - drug effects; Hybridomas - immunology; Lipopolysaccharides - pharmacology; Male; Mercaptoethanol - pharmacology; Mice; Mice, Inbred BALB C; Mitogens - pharmacology; Peritoneal Cavity - cytology

Peritoneal exudate cells from mice injected with immunostimulatory agents were evaluated for their ability to promote hybridoma growth. Peritoneal cells from mice receiving peritoneal injections of either Freund's incomplete adjuvant or pristane, seven days prior to harvesting, produced the gre...