Factors to consider in performing survival studies with insect cells

• Published in: In vitro cellular & developmental biology Vol. 23; no. 11; p. 733
• Main Author: Koval, T.M
• Format: Journal Article
• Language: English
• Published: United States 01.11.1987
• Subjects: Animals; BLOOD COMPOSITION; Blood Physiological Phenomena; Cattle; CELL CULTURE; Cell Division; Cells, Cultured - radiation effects; Colony-Forming Units Assay - methods; COMPOSICION DE LA SANGRE; COMPOSITION DU SANG; CULTIVO DE CELULAS; CULTURE DE CELLULES; CULTURE MEDIA; Culture Media - pharmacology; Diptera - cytology; INSECTE; INSECTOS; INSECTS; Lepidoptera - cytology; MEDIO DE CULTIVO; MILIEU DE CULTURE; SERUMS; SUPERVIVENCIA; SURVIE; SURVIVAL; TOXICIDAD; TOXICITE; TOXICITY; Ultraviolet Rays

Insect cell lines are not well-suited to colony formation in liquid medium following low-density cell plating. The present studies demonstrate that the time of addition of fetal bovine serum to the culture medium and the number of gamma-irradiated feeder cells added to each plate are important facto...