Integrated Whole-Cell Biocatalysis for Trehalose Production from Maltose Using Permeabilized Pseudomonas monteilii Cells and Bioremoval of Byproduct

• Published in: Journal of microbiology and biotechnology Vol. 32; no. 8; pp. 1054 - 1063
• Main Authors: Trakarnpaiboon, Srisakul, Champreda, Verawat
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Society for Microbiology and Biotechnology 28.08.2022; 한국미생물·생명공학회
• Subjects: Biocatalysis; Glucosyltransferases; Maltose - metabolism; Metabolic Engineering - methods; Pseudomonas; Research article; Trehalose - biosynthesis; 생물학; maltose; trehalose synthase; Pseudomonas monteilii; Trehalose; permeabilized cell

Trehalose is a non-conventional sugar with potent applications in the food, healthcare and biopharma industries. In this study, trehalose was synthesized from maltose using whole-cell TBRC 1196 producing trehalose synthase (TreS) as the biocatalyst. The reaction condition was optimized using 1% Trit...