MeDIP real-time qPCR of maternal peripheral blood reliably identifies trisomy 21

• Published in: Prenatal diagnosis Vol. 32; no. 10; pp. 996 - 1001
• Main Authors: Tsaliki, Evdokia, Papageorgiou, Elisavet A., Spyrou, Christiana, Koumbaris, George, Kypri, Elena, Kyriakou, Skevi, Sotiriou, Chrysovalanto, Touvana, Evi, Keravnou, Anna, Karagrigoriou, Alex, Lamnissou, Klea, Velissariou, Voula, Patsalis, Philippos C.
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.10.2012
• Subjects: Adult; DNA - blood; DNA Methylation - genetics; Down Syndrome - diagnosis; Down Syndrome - genetics; Female; Fetus - chemistry; Gestational Age; Humans; Immunosorbent Techniques; Pregnancy; Prenatal Diagnosis - methods; Real-Time Polymerase Chain Reaction - methods; Reproducibility of Results; Sensitivity and Specificity

ABSTRACT Objective To reevaluate the efficiency of the 12 differentially methylated regions (DMRs) used in the methylated DNA immunoprecipitation (MeDIP) real‐time quantitative polymerase chain reaction (real‐time qPCR) based approach, develop an improved version of the diagnostic formula and perfor...