A case report on death from acute bacterial cholangitis accompanied by von Meyenburg complexes: Use of 16S rRNA gene sequencing to identify pathogenic microbes from postmortem formalin-fixed, paraffin-embedded tissue

• Published in: Medicine (Baltimore) Vol. 100; no. 15; p. e25526
• Main Authors: Watanabe, Noriko, Ohno, Shin-ichiro, Sakuma, Moe, Kuriwaki, Mayo, Miura, Mai, Kuroda, Masahiko
• Format: Journal Article
• Language: English
• Published: United States Lippincott Williams & Wilkins 16.04.2021
• Subjects: Acute Disease; Aged, 80 and over; Autopsy; Biliary Tract Diseases - microbiology; Cholangitis - microbiology; Clinical Case Report; Fatal Outcome; Formaldehyde; Hamartoma - microbiology; Humans; Male; Medical Illustration; Paraffin Embedding; Pseudomonas aeruginosa; RNA, Bacterial - analysis; RNA, Ribosomal, 16S - analysis; Sequence Analysis, RNA

In some cases, autopsy is the first opportunity to find a previously unrecognized critical infection. Pathogens are identified by various methods, such as microscopic examination, special stains, culture tests, and immunohistochemistry. Here, we report a case of 16S ribosomal RNA (rRNA) gene sequenc...