Centenarians Overexpress Pluripotency-Related Genes
Human mesenchymal cells can become pluripotent by the addition of Yamanaka factors OCT3/4, SOX2, c-MYC, KLF4. We have recently reported that centenarians overexpress BCL-xL, which has been shown to improve pluripotency; thus, we aimed to determine the expression of pluripotency-related genes in cent...
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Published in | The journals of gerontology. Series A, Biological sciences and medical sciences Vol. 74; no. 9; p. 1391 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
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United States
16.08.2019
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Abstract | Human mesenchymal cells can become pluripotent by the addition of Yamanaka factors OCT3/4, SOX2, c-MYC, KLF4. We have recently reported that centenarians overexpress BCL-xL, which has been shown to improve pluripotency; thus, we aimed to determine the expression of pluripotency-related genes in centenarians. We recruited 22 young, 32 octogenarian, and 47 centenarian individuals and determined the mRNA expression of Yamanaka factors and other stemness-related cell surface marker genes (VIM, BMP4, NCAM, BMPR2) in peripheral blood mononuclear cells by reverse transcription polymerase chain reaction. We found that centenarians overexpress OCT3/4, SOX2, c-MYC, VIM, BMP4, NCAM, and BMPR2, when compared with octogenarians (p < .05). We further tested the functional role of BCL-xL in centenarians' ability to express pluripotency-related genes: lymphocytes from octogenarians transduced with BCL-xL overexpressed SOX2, c-MYC, and KLF4. We conclude that centenarians overexpress Yamanaka Factors and other stemness-related cell surface marker genes, which may contribute to their successful aging. |
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AbstractList | Human mesenchymal cells can become pluripotent by the addition of Yamanaka factors OCT3/4, SOX2, c-MYC, KLF4. We have recently reported that centenarians overexpress BCL-xL, which has been shown to improve pluripotency; thus, we aimed to determine the expression of pluripotency-related genes in centenarians. We recruited 22 young, 32 octogenarian, and 47 centenarian individuals and determined the mRNA expression of Yamanaka factors and other stemness-related cell surface marker genes (VIM, BMP4, NCAM, BMPR2) in peripheral blood mononuclear cells by reverse transcription polymerase chain reaction. We found that centenarians overexpress OCT3/4, SOX2, c-MYC, VIM, BMP4, NCAM, and BMPR2, when compared with octogenarians (p < .05). We further tested the functional role of BCL-xL in centenarians' ability to express pluripotency-related genes: lymphocytes from octogenarians transduced with BCL-xL overexpressed SOX2, c-MYC, and KLF4. We conclude that centenarians overexpress Yamanaka Factors and other stemness-related cell surface marker genes, which may contribute to their successful aging. |
Author | Viña, José Sanchís, Paula Mas-Bargues, Cristina Matheu, Ander Berna-Erro, Alejandro Avellana, Juan-Antonio Inglés, Marta Borrás, Consuelo |
Author_xml | – sequence: 1 givenname: Marta surname: Inglés fullname: Inglés, Marta organization: Freshage Research Group-Department of Physiotherapy, Faculty of Physiotherapy, University of Valencia, CIBERFES, INCLIVA, Spain – sequence: 2 givenname: Cristina surname: Mas-Bargues fullname: Mas-Bargues, Cristina organization: Freshage Research Group-Department of Physiology, Faculty of Medicine, University of Valencia, CIBERFES, INCLIVA, Spain – sequence: 3 givenname: Alejandro surname: Berna-Erro fullname: Berna-Erro, Alejandro organization: Cellular Oncology Department, Instituto Biodonostia, San Sebastian, Spain – sequence: 4 givenname: Ander surname: Matheu fullname: Matheu, Ander organization: Cellular Oncology Department, Instituto Biodonostia, San Sebastian, Spain – sequence: 5 givenname: Paula surname: Sanchís fullname: Sanchís, Paula organization: Geriatrics Department, Servicio de Geriatría, Hospital de la Ribera, Valencia, Spain – sequence: 6 givenname: Juan-Antonio surname: Avellana fullname: Avellana, Juan-Antonio organization: Geriatrics Department, Servicio de Geriatría, Hospital de la Ribera, Valencia, Spain – sequence: 7 givenname: Consuelo surname: Borrás fullname: Borrás, Consuelo organization: Freshage Research Group-Department of Physiology, Faculty of Medicine, University of Valencia, CIBERFES, INCLIVA, Spain – sequence: 8 givenname: José surname: Viña fullname: Viña, José organization: Freshage Research Group-Department of Physiology, Faculty of Medicine, University of Valencia, CIBERFES, INCLIVA, Spain |
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CitedBy_id | crossref_primary_10_1016_j_mad_2019_111199 crossref_primary_10_1097_MD_0000000000032746 crossref_primary_10_1093_gerona_glz132 crossref_primary_10_1038_s42003_021_02254_3 crossref_primary_10_1093_gerona_glac119 crossref_primary_10_3390_ijms24032888 crossref_primary_10_1038_s43587_023_00527_6 crossref_primary_10_1186_s12877_023_04159_8 crossref_primary_10_1016_j_mad_2024_111936 crossref_primary_10_1016_j_freeradbiomed_2020_11_026 crossref_primary_10_1111_acel_14201 crossref_primary_10_1002_1873_3468_14963 crossref_primary_10_1016_j_arr_2021_101392 |
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Copyright | The Author(s) 2018. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. |
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Keywords | Stemness Longevity Healthy aging Pluripotency |
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License | The Author(s) 2018. Published by Oxford University Press on behalf of The Gerontological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. |
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Snippet | Human mesenchymal cells can become pluripotent by the addition of Yamanaka factors OCT3/4, SOX2, c-MYC, KLF4. We have recently reported that centenarians... |
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Title | Centenarians Overexpress Pluripotency-Related Genes |
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