Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers

• Published in: Journal of visualized experiments Vol. 2021; no. 169
• Main Authors: Zhou, Mowei, Abdali, Shadan H, Dilworth, David, Liu, Lifeng, Cole, Benjamin, Malhan, Neha, Ahkami, Amir H, Winkler, Tanya E, Hollingsworth, Joy, Sievert, Julie, Dahlberg, Jeff, Hutmacher, Robert, Madera, Mary, Owiti, Judith A, Hixson, Kim K, Lemaux, Peggy G, Jansson, Christer, Paša-Tolić, Ljiljana
• Format: Journal Article
• Language: English
• Published: United States MyJoVE Corp 04.03.2021
• Subjects: Amino Acid Sequence; Biomarkers - metabolism; Buffers; Cell Nucleus - metabolism; Chromatography, Liquid; Epigenesis, Genetic; Histones - chemistry; Histones - isolation & purification; Histones - metabolism; Mass Spectrometry; Plant Leaves - metabolism; Plant Proteins - chemistry; Plant Proteins - isolation & purification; Plant Proteins - metabolism; Protein Processing, Post-Translational; Sorghum - genetics; Sorghum - metabolism

Histones belong to a family of highly conserved proteins in eukaryotes. They pack DNA into nucleosomes as functional units of chromatin. Post-translational modifications (PTMs) of histones, which are highly dynamic and can be added or removed by enzymes, play critical roles in regulating gene expres...