Efficient construction of a highly useful phage-displayed human antibody repertoire

• Published in: Biochemical and biophysical research communications Vol. 336; no. 4; pp. 1207 - 1213
• Main Authors: Rojas, Gertrudis, Lamdan, Humberto, Padron, Sheila, Munoz, Yasmiana, Ayala, Marta, Gavilondo, Jorge V.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 04.11.2005
• Subjects: Amino Acid Sequence; Antibody library; Antigens - metabolism; Humans; Immunoglobulin Fragments - genetics; Immunoglobulin Fragments - metabolism; Immunoglobulin Variable Region - genetics; Lymphocytes - metabolism; Molecular Sequence Data; Peptide Library; Phage display; Protein Binding; Repertoire size; Surface Plasmon Resonance; Antibody library; Phage display; Repertoire size

We have constructed a highly useful phage-displayed human antibody repertoire with limited cloning efforts. Our strategy was to maximize diversity during the first steps of library construction through the use of various lymphoid sources from several donors, inclusion of different immunoglobulin iso...