Assessment of sarcomere shortening and calcium transient in primary human and dog ventricular myocytes

• Published in: Journal of pharmacological and toxicological methods Vol. 123; p. 107278
• Main Authors: Gao, BaoXi, Abi-Gerges, Najah, Truong, Ky, Stafford, Alexa, Nguyen, William, Sutherland, Weston, Vargas, Hugo M., Qu, Yusheng
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2023
• Subjects: Calcium transients; Dog myocytes; Human myocytes; Inotropes; Isolated myocytes; Sarcomere shortening; Cont-P; Inotropes; ISO; Dog myocytes; CaT; Cont-Dur; CTD50; Calcium transients; Sarcomere shortening; CTAs; Human myocytes; Isolated myocytes

Understanding translation from preclinical observations to clinical findings is important for evaluating the efficacy and safety of novel compounds. Of relevance to cardiac safety is profiling drug effects on cardiomyocyte (CM) sarcomere shortening and intracellular Ca2+ dynamics. Although CM from d...