Estradiol inhibits transcription of the human glycoprotein hormone α-subunit gene despite the absence of a high affinity binding site for estrogen receptor

• Published in: Molecular endocrinology (Baltimore, Md.) Vol. 5; no. 5; pp. 725 - 733
• Main Authors: KERI, R. A, ANDERSEN, B, KENNEDY, G. C, HAMERNIK, D. L, CLAY, C. M, BRACE, A. D, NETT, T. M, NOTIDES, A. C, NILSON, J. H
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.05.1991
• Subjects: Animals; Biological and medical sciences; Cattle; Cell Line; Chimera; Chloramphenicol O-Acetyltransferase - genetics; Estradiol - pharmacology; Female; Fundamental and applied biological sciences. Psychology; Gene expression; Genes; Glycoprotein Hormones, alpha Subunit - genetics; Glycoprotein Hormones, alpha Subunit - metabolism; Humans; Mice; Mice, Transgenic; Molecular and cellular biology; Molecular genetics; Organ Specificity; Promoter Regions, Genetic; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Transcription, Genetic - drug effects; Transfection; Transcription promoter; Estrogen; Transgenic animal; Molecular interaction; Binding site; Gene expression; Glycoprotein hormone; Estradiol; Cell line; Adenohypophyseal hormone; Hormonal regulation; Sex steroid hormone; Hybrid gene; Hormonal receptor

Chronic administration of estradiol inhibits transcription of the gene encoding the alpha-subunit of pituitary glycoprotein hormones. Here, we show, using transfection analyses and a filter binding assay, that 1500 basepairs of proximal 5' flanking sequence of the human alpha-subunit gene lack...