Reduced TCA flux in diabetic myotubes: A governing influence on the diabetic phenotype?
The diabetic phenotype is complex, requiring elucidation of key initiating defects. It is unknown whether the reduced tricarboxylic acid cycle (TCA) flux in skeletal muscle of obese and obese type 2 diabetic (T2D) subjects is of primary origin. Acetate oxidation (measurement of TCA-flux) was signifi...
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| Published in | Biochemical and biophysical research communications Vol. 387; no. 4; pp. 651 - 655 |
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| Format | Journal Article |
| Language | English |
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Elsevier Inc
02.10.2009
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| Abstract | The diabetic phenotype is complex, requiring elucidation of key initiating defects. It is unknown whether the reduced tricarboxylic acid cycle (TCA) flux in skeletal muscle of obese and obese type 2 diabetic (T2D) subjects is of primary origin. Acetate oxidation (measurement of TCA-flux) was significantly reduced in primary myotube cultures established from T2D versus lean subjects. Acetate oxidation was acutely stimulated by insulin and respiratory uncoupling. Inhibition of TCA flux in lean myotubes by malonate was followed by a measured decline in; acetate oxidation, complete palmitate oxidation, lipid uptake, glycogen synthesis, ATP content and increased glucose uptake, while glucose oxidation was unaffected. Acute TCA inhibition did not induce insulin resistance. Thus the reduced TCA cycle flux in T2D skeletal muscle may be of primary origin. The diabetic phenotype of increased basal glucose uptake and glucose oxidation, the reduced complete lipid oxidation and increased respiratory quotient, are likely to be adaptive responses to the reduced TCA cycle flux. |
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| AbstractList | The diabetic phenotype is complex, requiring elucidation of key initiating defects. It is unknown whether the reduced tricarboxylic acid cycle (TCA) flux in skeletal muscle of obese and obese type 2 diabetic (T2D) subjects is of primary origin. Acetate oxidation (measurement of TCA-flux) was significantly reduced in primary myotube cultures established from T2D versus lean subjects. Acetate oxidation was acutely stimulated by insulin and respiratory uncoupling. Inhibition of TCA flux in lean myotubes by malonate was followed by a measured decline in; acetate oxidation, complete palmitate oxidation, lipid uptake, glycogen synthesis, ATP content and increased glucose uptake, while glucose oxidation was unaffected. Acute TCA inhibition did not induce insulin resistance. Thus the reduced TCA cycle flux in T2D skeletal muscle may be of primary origin. The diabetic phenotype of increased basal glucose uptake and glucose oxidation, the reduced complete lipid oxidation and increased respiratory quotient, are likely to be adaptive responses to the reduced TCA cycle flux. The diabetic phenotype is complex, requiring elucidation of key initiating defects. It is unknown whether the reduced tricarboxylic acid cycle (TCA) flux in skeletal muscle of obese and obese type 2 diabetic (T2D) subjects is of primary origin. Acetate oxidation (measurement of TCA-flux) was significantly reduced in primary myotube cultures established from T2D versus lean subjects. Acetate oxidation was acutely stimulated by insulin and respiratory uncoupling. Inhibition of TCA flux in lean myotubes by malonate was followed by a measured decline in; acetate oxidation, complete palmitate oxidation, lipid uptake, glycogen synthesis, ATP content and increased glucose uptake, while glucose oxidation was unaffected. Acute TCA inhibition did not induce insulin resistance. Thus the reduced TCA cycle flux in T2D skeletal muscle may be of primary origin. The diabetic phenotype of increased basal glucose uptake and glucose oxidation, the reduced complete lipid oxidation and increased respiratory quotient, are likely to be adaptive responses to the reduced TCA cycle flux.The diabetic phenotype is complex, requiring elucidation of key initiating defects. It is unknown whether the reduced tricarboxylic acid cycle (TCA) flux in skeletal muscle of obese and obese type 2 diabetic (T2D) subjects is of primary origin. Acetate oxidation (measurement of TCA-flux) was significantly reduced in primary myotube cultures established from T2D versus lean subjects. Acetate oxidation was acutely stimulated by insulin and respiratory uncoupling. Inhibition of TCA flux in lean myotubes by malonate was followed by a measured decline in; acetate oxidation, complete palmitate oxidation, lipid uptake, glycogen synthesis, ATP content and increased glucose uptake, while glucose oxidation was unaffected. Acute TCA inhibition did not induce insulin resistance. Thus the reduced TCA cycle flux in T2D skeletal muscle may be of primary origin. The diabetic phenotype of increased basal glucose uptake and glucose oxidation, the reduced complete lipid oxidation and increased respiratory quotient, are likely to be adaptive responses to the reduced TCA cycle flux. |
| Author | Gaster, Michael |
| Author_xml | – sequence: 1 givenname: Michael surname: Gaster fullname: Gaster, Michael email: Michael.Gaster@ouh.fyns-amt.dk organization: KMEB, Dept. of Endocrinology, Odense University Hospital, Sdr Boulevard, DK-5000 Odense, Denmark |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/19615969$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1007/s00125-008-1122-9 10.1038/ng1180 10.1016/j.bbadis.2005.04.001 10.1373/clinchem.2005.062414 10.1042/CS19990328 10.1034/j.1600-0463.2001.d01-140.x 10.1172/JCI117600 10.1016/j.bbrc.2009.03.102 10.1016/S0925-4439(01)00071-0 10.1194/jlr.M600319-JLR200 10.1073/pnas.1332551100 10.1016/j.bbalip.2005.12.003 10.2337/db06-0783 10.1016/j.bbadis.2004.05.006 10.2337/diabetes.49.5.677 10.2337/diabetes.53.3.542 10.1016/j.bbadis.2007.03.007 10.1371/journal.pmed.0020233 10.1073/pnas.1032913100 10.1007/s00125-008-1069-x 10.2337/diabetes.51.4.921 10.1111/j.1748-1716.2009.01975.x |
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| Keywords | CS Acetate oxidation PC TCA ARF T2D OXPHOS OAA |
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| References | Passonneau, Lowry (bib17) 1993 Schrauwen, Blaak, Aggel-Leijssen, Borghouts, Wagenmakers (bib18) 2000; 98 Janssen, Trijbels, Sengers, Wintjes, Ruitenbeek, Smeitink, Morava, van Engelen, van den Heuvel, Rodenburg (bib16) 2006; 52 McBride, Hardie (bib24) 2009; 196 Gaster (bib19) 2007; 48 Sidossis, Coggan, Gastaldelli, Wolfe (bib6) 1995; 269 Schrauwen, Hesselink (bib5) 2008; 51 Gaster, Beck-Nielsen (bib10) 2004; 1690 Gaster, Petersen, Hojlund, Poulsen, Beck-Nielsen (bib8) 2002; 51 Gaster (bib15) 2007; 1772 Patti, Butte, Crunkhorn, Cusi, Berria, Kashyap, Miyazaki, Kohane, Costello, Saccone, Landaker, Goldfine, Mun, DeFronzo, Finlayson, Kahn, Mandarino (bib2) 2003; 100 Gaster, Kristensen, Beck-Nielsen, Schroder (bib13) 2001; 109 Gaster (bib11) 2009; 382 Gaster, Rustan, Aas, Beck-Nielsen (bib9) 2004; 53 Gaster, Beck-Nielsen (bib12) 2006; 1761 Petersen, Dufour, Shulman (bib23) 2005; 2 Befroy, Petersen, Dufour, Mason, de Graaf, Rothman, Shulman (bib4) 2007; 56 Mootha, Lindgren, Eriksson, Subramanian, Sihag, Lehar, Puigserver, Carlsson, Ridderstrale, Laurila, Houstis, Daly, Patterson, Mesirov, Golub, Tamayo, Spiegelman, Lander, Hirschhorn, Altshuler, Groop (bib1) 2003; 34 Gaster, Schroder, Handberg, Beck-Nielsen (bib14) 2001; 1537 Kelley, Simoneau (bib21) 1994; 94 Frederiksen, Hojlund, Hansen, Oakeley, Hemmings, Abdallah, Brusgaard, Beck-Nielsen, Gaster (bib3) 2008; 51 Ortenblad, Mogensen, Petersen, Hojlund, Levin, Sahlin, Beck-Nielsen, Gaster (bib7) 2005; 1741 Stump, Short, Bigelow, Schimke, Nair (bib22) 2003; 100 Kelley, Mandarino (bib20) 2000; 49 Gaster (10.1016/j.bbrc.2009.07.064_bib8) 2002; 51 Gaster (10.1016/j.bbrc.2009.07.064_bib15) 2007; 1772 Patti (10.1016/j.bbrc.2009.07.064_bib2) 2003; 100 Sidossis (10.1016/j.bbrc.2009.07.064_bib6) 1995; 269 Schrauwen (10.1016/j.bbrc.2009.07.064_bib18) 2000; 98 Ortenblad (10.1016/j.bbrc.2009.07.064_bib7) 2005; 1741 Gaster (10.1016/j.bbrc.2009.07.064_bib12) 2006; 1761 Gaster (10.1016/j.bbrc.2009.07.064_bib9) 2004; 53 Mootha (10.1016/j.bbrc.2009.07.064_bib1) 2003; 34 Gaster (10.1016/j.bbrc.2009.07.064_bib11) 2009; 382 Frederiksen (10.1016/j.bbrc.2009.07.064_bib3) 2008; 51 McBride (10.1016/j.bbrc.2009.07.064_bib24) 2009; 196 Gaster (10.1016/j.bbrc.2009.07.064_bib19) 2007; 48 Befroy (10.1016/j.bbrc.2009.07.064_bib4) 2007; 56 Kelley (10.1016/j.bbrc.2009.07.064_bib20) 2000; 49 Gaster (10.1016/j.bbrc.2009.07.064_bib10) 2004; 1690 Passonneau (10.1016/j.bbrc.2009.07.064_bib17) 1993 Petersen (10.1016/j.bbrc.2009.07.064_bib23) 2005; 2 Gaster (10.1016/j.bbrc.2009.07.064_bib14) 2001; 1537 Janssen (10.1016/j.bbrc.2009.07.064_bib16) 2006; 52 Stump (10.1016/j.bbrc.2009.07.064_bib22) 2003; 100 Schrauwen (10.1016/j.bbrc.2009.07.064_bib5) 2008; 51 Gaster (10.1016/j.bbrc.2009.07.064_bib13) 2001; 109 Kelley (10.1016/j.bbrc.2009.07.064_bib21) 1994; 94 |
| References_xml | – volume: 100 start-page: 8466 year: 2003 end-page: 8471 ident: bib2 article-title: Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: potential role of PGC1 and NRF1 publication-title: Proc. Natl. Acad. Sci. USA – volume: 1772 start-page: 755 year: 2007 end-page: 765 ident: bib15 article-title: Insulin resistance and the mitochondrial link. Lessons from cultured human myotubes publication-title: Biochim. Biophys. Acta – volume: 98 start-page: 587 year: 2000 end-page: 592 ident: bib18 article-title: Determinants of the acetate recovery factor: implications for estimation of [ publication-title: Clin. Sci. (Lond.) – volume: 56 start-page: 1376 year: 2007 end-page: 1381 ident: bib4 article-title: Impaired mitochondrial substrate oxidation in muscle of insulin-resistant offspring of type 2 diabetic patients publication-title: Diabetes – volume: 51 start-page: 1694 year: 2008 end-page: 1697 ident: bib5 article-title: Reduced tricarboxylic acid cycle flux in type 2 diabetes mellitus? publication-title: Diabetologia – volume: 1690 start-page: 85 year: 2004 end-page: 91 ident: bib10 article-title: The reduced insulin mediated glucose oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin – evidence from cultured myotubes publication-title: Biochim. Biophys. Acta – volume: 100 start-page: 7996 year: 2003 end-page: 8001 ident: bib22 article-title: Effect of insulin on human skeletal muscle mitochondrial ATP production, protein synthesis, and mRNA transcripts publication-title: Proc. Natl. Acad. Sci. USA – volume: 1537 start-page: 211 year: 2001 end-page: 221 ident: bib14 article-title: The basal kinetic parameters of glycogen synthase in human myotube cultures are not affected by chronic high insulin exposure publication-title: Biochim. Biophys. Acta – volume: 34 start-page: 267 year: 2003 end-page: 273 ident: bib1 article-title: PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes publication-title: Nat. Genet. – volume: 51 start-page: 921 year: 2002 end-page: 927 ident: bib8 article-title: The diabetic phenotype is conserved in myotubes established from diabetic subjects: evidence for primary defects in glucose transport and glycogen synthase activity publication-title: Diabetes – volume: 52 start-page: 860 year: 2006 end-page: 871 ident: bib16 article-title: Measurement of the energy-generating capacity of human muscle mitochondria: diagnostic procedure and application to human pathology publication-title: Clin. Chem. – volume: 382 start-page: 766 year: 2009 end-page: 770 ident: bib11 article-title: Reduced lipid oxidation in myotubes established from obese and type 2 diabetic subjects publication-title: Biochem. Biophys. Res. Commun. – volume: 53 start-page: 542 year: 2004 end-page: 548 ident: bib9 article-title: Reduced lipid oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin: evidence from cultured myotubes publication-title: Diabetes – volume: 1741 start-page: 206 year: 2005 end-page: 214 ident: bib7 article-title: Reduced insulin-mediated citrate synthase activity in cultured skeletal muscle cells from patients with type 2 diabetes: evidence for an intrinsic oxidative enzyme defect publication-title: Biochim. Biophys. Acta – volume: 51 start-page: 2068 year: 2008 end-page: 2077 ident: bib3 article-title: Transcriptional profiling of myotubes from patients with type 2 diabetes: no evidence for a primary defect in oxidative phosphorylation genes publication-title: Diabetologia – volume: 1761 start-page: 100 year: 2006 end-page: 110 ident: bib12 article-title: Triacylglycerol accumulation is not primarily affected in myotubes established from type 2 diabetic subjects publication-title: Biochim. Biophys. Acta – volume: 48 start-page: 207 year: 2007 end-page: 217 ident: bib19 article-title: Metabolic flexibility is conserved in diabetic myotubes publication-title: J. Lipid Res. – volume: 2 start-page: e233 year: 2005 ident: bib23 article-title: Decreased insulin-stimulated ATP synthesis and phosphate transport in muscle of insulin-resistant offspring of type 2 diabetic parents publication-title: PLoS. Med. – volume: 94 start-page: 2349 year: 1994 end-page: 2356 ident: bib21 article-title: Impaired free fatty acid utilization by skeletal muscle in non-insulin-dependent diabetes mellitus publication-title: J. Clin. Invest. – volume: 269 start-page: E649 year: 1995 end-page: E656 ident: bib6 article-title: A new correction factor for use in tracer estimations of plasma fatty acid oxidation publication-title: Am. J. Physiol. – volume: 49 start-page: 677 year: 2000 end-page: 683 ident: bib20 article-title: Fuel selection in human skeletal muscle in insulin resistance: a reexamination publication-title: Diabetes – year: 1993 ident: bib17 article-title: Enzymatic Analysis: A Practical Guide – volume: 196 start-page: 99 year: 2009 end-page: 113 ident: bib24 article-title: AMP-activated protein kinase – a sensor of glycogen as well as AMP and ATP? publication-title: Acta Physiol. (Oxford) – volume: 109 start-page: 735 year: 2001 end-page: 744 ident: bib13 article-title: A cellular model system of differentiated human myotubes publication-title: APMIS – volume: 51 start-page: 2068 year: 2008 ident: 10.1016/j.bbrc.2009.07.064_bib3 article-title: Transcriptional profiling of myotubes from patients with type 2 diabetes: no evidence for a primary defect in oxidative phosphorylation genes publication-title: Diabetologia doi: 10.1007/s00125-008-1122-9 – volume: 34 start-page: 267 year: 2003 ident: 10.1016/j.bbrc.2009.07.064_bib1 article-title: PGC-1alpha-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes publication-title: Nat. Genet. doi: 10.1038/ng1180 – volume: 1741 start-page: 206 year: 2005 ident: 10.1016/j.bbrc.2009.07.064_bib7 article-title: Reduced insulin-mediated citrate synthase activity in cultured skeletal muscle cells from patients with type 2 diabetes: evidence for an intrinsic oxidative enzyme defect publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbadis.2005.04.001 – volume: 52 start-page: 860 year: 2006 ident: 10.1016/j.bbrc.2009.07.064_bib16 article-title: Measurement of the energy-generating capacity of human muscle mitochondria: diagnostic procedure and application to human pathology publication-title: Clin. Chem. doi: 10.1373/clinchem.2005.062414 – volume: 98 start-page: 587 year: 2000 ident: 10.1016/j.bbrc.2009.07.064_bib18 article-title: Determinants of the acetate recovery factor: implications for estimation of [13C]substrate oxidation publication-title: Clin. Sci. (Lond.) doi: 10.1042/CS19990328 – volume: 109 start-page: 735 year: 2001 ident: 10.1016/j.bbrc.2009.07.064_bib13 article-title: A cellular model system of differentiated human myotubes publication-title: APMIS doi: 10.1034/j.1600-0463.2001.d01-140.x – volume: 94 start-page: 2349 year: 1994 ident: 10.1016/j.bbrc.2009.07.064_bib21 article-title: Impaired free fatty acid utilization by skeletal muscle in non-insulin-dependent diabetes mellitus publication-title: J. Clin. Invest. doi: 10.1172/JCI117600 – year: 1993 ident: 10.1016/j.bbrc.2009.07.064_bib17 – volume: 382 start-page: 766 year: 2009 ident: 10.1016/j.bbrc.2009.07.064_bib11 article-title: Reduced lipid oxidation in myotubes established from obese and type 2 diabetic subjects publication-title: Biochem. Biophys. Res. Commun. doi: 10.1016/j.bbrc.2009.03.102 – volume: 1537 start-page: 211 year: 2001 ident: 10.1016/j.bbrc.2009.07.064_bib14 article-title: The basal kinetic parameters of glycogen synthase in human myotube cultures are not affected by chronic high insulin exposure publication-title: Biochim. Biophys. Acta doi: 10.1016/S0925-4439(01)00071-0 – volume: 48 start-page: 207 year: 2007 ident: 10.1016/j.bbrc.2009.07.064_bib19 article-title: Metabolic flexibility is conserved in diabetic myotubes publication-title: J. Lipid Res. doi: 10.1194/jlr.M600319-JLR200 – volume: 100 start-page: 7996 year: 2003 ident: 10.1016/j.bbrc.2009.07.064_bib22 article-title: Effect of insulin on human skeletal muscle mitochondrial ATP production, protein synthesis, and mRNA transcripts publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1332551100 – volume: 1761 start-page: 100 year: 2006 ident: 10.1016/j.bbrc.2009.07.064_bib12 article-title: Triacylglycerol accumulation is not primarily affected in myotubes established from type 2 diabetic subjects publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbalip.2005.12.003 – volume: 56 start-page: 1376 year: 2007 ident: 10.1016/j.bbrc.2009.07.064_bib4 article-title: Impaired mitochondrial substrate oxidation in muscle of insulin-resistant offspring of type 2 diabetic patients publication-title: Diabetes doi: 10.2337/db06-0783 – volume: 269 start-page: E649 year: 1995 ident: 10.1016/j.bbrc.2009.07.064_bib6 article-title: A new correction factor for use in tracer estimations of plasma fatty acid oxidation publication-title: Am. J. Physiol. – volume: 1690 start-page: 85 year: 2004 ident: 10.1016/j.bbrc.2009.07.064_bib10 article-title: The reduced insulin mediated glucose oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin – evidence from cultured myotubes publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbadis.2004.05.006 – volume: 49 start-page: 677 year: 2000 ident: 10.1016/j.bbrc.2009.07.064_bib20 article-title: Fuel selection in human skeletal muscle in insulin resistance: a reexamination publication-title: Diabetes doi: 10.2337/diabetes.49.5.677 – volume: 53 start-page: 542 year: 2004 ident: 10.1016/j.bbrc.2009.07.064_bib9 article-title: Reduced lipid oxidation in skeletal muscle from type 2 diabetic subjects may be of genetic origin: evidence from cultured myotubes publication-title: Diabetes doi: 10.2337/diabetes.53.3.542 – volume: 1772 start-page: 755 year: 2007 ident: 10.1016/j.bbrc.2009.07.064_bib15 article-title: Insulin resistance and the mitochondrial link. Lessons from cultured human myotubes publication-title: Biochim. Biophys. Acta doi: 10.1016/j.bbadis.2007.03.007 – volume: 2 start-page: e233 year: 2005 ident: 10.1016/j.bbrc.2009.07.064_bib23 article-title: Decreased insulin-stimulated ATP synthesis and phosphate transport in muscle of insulin-resistant offspring of type 2 diabetic parents publication-title: PLoS. Med. doi: 10.1371/journal.pmed.0020233 – volume: 100 start-page: 8466 year: 2003 ident: 10.1016/j.bbrc.2009.07.064_bib2 article-title: Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: potential role of PGC1 and NRF1 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1032913100 – volume: 51 start-page: 1694 year: 2008 ident: 10.1016/j.bbrc.2009.07.064_bib5 article-title: Reduced tricarboxylic acid cycle flux in type 2 diabetes mellitus? publication-title: Diabetologia doi: 10.1007/s00125-008-1069-x – volume: 51 start-page: 921 year: 2002 ident: 10.1016/j.bbrc.2009.07.064_bib8 article-title: The diabetic phenotype is conserved in myotubes established from diabetic subjects: evidence for primary defects in glucose transport and glycogen synthase activity publication-title: Diabetes doi: 10.2337/diabetes.51.4.921 – volume: 196 start-page: 99 year: 2009 ident: 10.1016/j.bbrc.2009.07.064_bib24 article-title: AMP-activated protein kinase – a sensor of glycogen as well as AMP and ATP? publication-title: Acta Physiol. (Oxford) doi: 10.1111/j.1748-1716.2009.01975.x |
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| Snippet | The diabetic phenotype is complex, requiring elucidation of key initiating defects. It is unknown whether the reduced tricarboxylic acid cycle (TCA) flux in... |
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| SubjectTerms | Acetate oxidation Citric Acid Cycle Diabetes Mellitus, Type 2 - metabolism Human Humans Middle Aged Muscle Fibers, Skeletal - metabolism Myotubes Phenotype Skeletal muscle Tricarboxylic acid cycle Type 2 diabetes |
| Title | Reduced TCA flux in diabetic myotubes: A governing influence on the diabetic phenotype? |
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