Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa
The causative molecular pathways underlying the pathogenesis of colorectal cancer (CRC) need to be better characterized. The purpose of our study was to better understand the genetic mechanism of oncogenesis for human colorectal cancer and to identify new potential tumor markers of use in clinical p...
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Published in | International journal of oncology Vol. 29; no. 1; pp. 83 - 94 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Athens
Editorial Academy of the International Journal of Oncology
01.07.2006
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Subjects | |
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Abstract | The causative molecular pathways underlying the pathogenesis of colorectal cancer (CRC) need to be better characterized. The purpose of our study was to better understand the genetic mechanism of oncogenesis for human colorectal cancer and to identify new potential tumor markers of use in clinical practice. We used cDNA microarrays to compare gene expression profiles of colorectal biopsies from 25 CRC patients and 13 normal mucosa from adjacent non-cancerous tissues. Findings were validated by real-time PCR; in addition, western blotting and immunochemistry analysis were carried out as further confirmation of differential expression at a protein level. Comparing cancerous tissues with normal colonic mucosa we identified 584 known genes differentially expressed to a significant degree (p<0.001). Many of the transcripts that were more abundant in tumors than in non-neoplastic tissues appear to reflect important events for colon carcinogenesis. For example, a significant number of these genes serve as apoptotic inhibitors (e.g. BFAR, BIRC1, BIRC6). Furthermore, we observed the simultaneous up-regulation of HLA-E and the down-regulation of beta2-microglobulin; these genes strongly support a potential tumor escape strategy from immune surveillance in colon cancer tissues. Our study provides new gene candidates in the pathogenesis of human CRC disease. From our results we hypothesize that CRC cells escape immune surveillance through a specific gene expression alteration; moreover, over-expression of several survival genes seems to confer a more anti-apoptotic phenotype. These genes are involved in pathways not previously implicated in CRC pathogenesis and they may provide new targets for therapy. |
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AbstractList | The causative molecular pathways underlying the pathogenesis of colorectal cancer (CRC) need to be better characterized. The purpose of our study was to better understand the genetic mechanism of oncogenesis for human colorectal cancer and to identify new potential tumor markers of use in clinical practice. We used cDNA microarrays to compare gene expression profiles of colorectal biopsies from 25 CRC patients and 13 normal mucosa from adjacent non-cancerous tissues. Findings were validated by real-time PCR; in addition, western blotting and immunochemistry analysis were carried out as further confirmation of differential expression at a protein level. Comparing cancerous tissues with normal colonic mucosa we identified 584 known genes differentially expressed to a significant degree (p<0.001). Many of the transcripts that were more abundant in tumors than in non-neoplastic tissues appear to reflect important events for colon carcinogenesis. For example, a significant number of these genes serve as apoptotic inhibitors (e.g. BFAR, BIRC1, BIRC6). Furthermore, we observed the simultaneous up-regulation of HLA-E and the down-regulation of beta2-microglobulin; these genes strongly support a potential tumor escape strategy from immune surveillance in colon cancer tissues. Our study provides new gene candidates in the pathogenesis of human CRC disease. From our results we hypothesize that CRC cells escape immune surveillance through a specific gene expression alteration; moreover, over-expression of several survival genes seems to confer a more anti-apoptotic phenotype. These genes are involved in pathways not previously implicated in CRC pathogenesis and they may provide new targets for therapy. |
Author | ZUCCHINI, Cinzia VALVASSORI, Luisa MORDOH, José LEVY, Estrella DE SANCTIS, Paola BIANCHINI, Michele PINSKI, Victor MACAGNO, Carlos CARINCI, Paolo |
Author_xml | – sequence: 1 givenname: Michele surname: BIANCHINI fullname: BIANCHINI, Michele organization: Centro de Investigaciones Oncológicas (CIO-FUCA), Universidad de Buenos Aires, Argentina – sequence: 2 givenname: Estrella surname: LEVY fullname: LEVY, Estrella organization: Centro de Investigaciones Oncológicas (CIO-FUCA), Universidad de Buenos Aires, Argentina – sequence: 3 givenname: Cinzia surname: ZUCCHINI fullname: ZUCCHINI, Cinzia organization: Dipartimento di Istologia, Embriologia e Biologia Applicata, Università degli Studi di Bologna, Via Belmeloro 8, 40126 Bologna, Italy – sequence: 4 givenname: Victor surname: PINSKI fullname: PINSKI, Victor organization: Maestría en Biología Molecular Médica, Universidad de Buenos Aires, Argentina – sequence: 5 givenname: Carlos surname: MACAGNO fullname: MACAGNO, Carlos organization: Maestría en Biología Molecular Médica, Universidad de Buenos Aires, Argentina – sequence: 6 givenname: Paola surname: DE SANCTIS fullname: DE SANCTIS, Paola organization: Dipartimento di Istologia, Embriologia e Biologia Applicata, Università degli Studi di Bologna, Via Belmeloro 8, 40126 Bologna, Italy – sequence: 7 givenname: Luisa surname: VALVASSORI fullname: VALVASSORI, Luisa organization: Dipartimento di Istologia, Embriologia e Biologia Applicata, Università degli Studi di Bologna, Via Belmeloro 8, 40126 Bologna, Italy – sequence: 8 givenname: Paolo surname: CARINCI fullname: CARINCI, Paolo organization: Dipartimento di Istologia, Embriologia e Biologia Applicata, Università degli Studi di Bologna, Via Belmeloro 8, 40126 Bologna, Italy – sequence: 9 givenname: José surname: MORDOH fullname: MORDOH, José organization: Laboratorio de Cancerología Fundacion Instituto Leloir, Argentina |
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Keywords | Human anti-apoptotic phenotype Rectal disease Mucosa Colorectal cancer cDNA microarray Malignant tumor Gene expression Gene expression profile Colonic disease Phenotype Colon cancer Cancerology Complementary DNA Gene Tissue microarrays gene expression profiles Digestive diseases Intestinal disease Comparative study Apoptosis |
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SubjectTerms | Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - metabolism Adult Aged Aged, 80 and over Apoptosis Regulatory Proteins - genetics Apoptosis Regulatory Proteins - metabolism beta 2-Microglobulin - genetics beta 2-Microglobulin - metabolism Biological and medical sciences Colorectal Neoplasms - genetics Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Female Gastroenterology. Liver. Pancreas. Abdomen Gene Expression Profiling - methods Gene Expression Regulation, Neoplastic Genes, Neoplasm Histocompatibility Antigens Class I - genetics Histocompatibility Antigens Class I - metabolism HLA Antigens - genetics HLA Antigens - metabolism HLA-E Antigens Humans Intestinal Mucosa - metabolism Male Medical sciences Membrane Proteins - genetics Membrane Proteins - metabolism Middle Aged Neuronal Apoptosis-Inhibitory Protein - genetics Neuronal Apoptosis-Inhibitory Protein - metabolism Oligonucleotide Array Sequence Analysis - methods Reproducibility of Results RNA, Messenger - analysis RNA, Messenger - metabolism Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Tumors |
Title | Comparative study of gene expression by cDNA microarray in human colorectal cancer tissues and normal mucosa |
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