Antimicrobial Efficacy of a Portable UV-C-Based Coating Activation Device against Candida albicans Biofilm and SARS-CoV-2 as an Additional Feature: An In Vitro Study

This in vitro study aimed to confirm the effect of the additional features of Kirei Keep Light (KKL), a commercial UV-C irradiation system that was originally created for coating the surface of removable dentures with photoreactive 2-methacryloyloxyethyl phosphorylcholine (MPC), on the antimicrobial...

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Published inHygiene Vol. 4; no. 1; pp. 93 - 102
Main Authors Putra Wigianto, Adityakrisna Yoshi, Watanabe, Megumi, Iwawaki, Yuki, Goto, Takaharu, Otsuki, Tamaki, Ichikawa, Tetsuo
Format Journal Article
LanguageEnglish
Japanese
Published MDPI AG 18.02.2024
Subjects
Candida albicans
HD7260-7780.8
Industrial hygiene. Industrial welfare
Industrial medicine. Industrial hygiene
MPC coating
RC963-969
SARS-CoV-2
UV-C irradiation
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ISSN2673-947X
2673-947X
DOI10.3390/hygiene4010006

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Summary:This in vitro study aimed to confirm the effect of the additional features of Kirei Keep Light (KKL), a commercial UV-C irradiation system that was originally created for coating the surface of removable dentures with photoreactive 2-methacryloyloxyethyl phosphorylcholine (MPC), on the antimicrobial efficacy aspect against Candida albicans biofilm on a denture base material (PMMA) and SARS-CoV-2. Materials and Methods: The antimicrobial efficacy on C. albicans biofilm was evaluated through quantitative (CFU) and qualitative (SEM images) analysis of three groups: no treatment (control), KKL, and immersion in a disinfectant solution, MCAE. The quantitative evaluation on SARS-CoV-2 was performed by comparing the untreated (control) group and the KKL group. Results: In comparison with the control group (2.39 × 106 CFU/mL), KKL irradiation resulted in a 91.01% reduction in C. albicans biofilm (2.15 × 105 CFU/mL), whereas for the MCAE group, this reduction was 99.98% (4.64 × 102 CFU/mL). The SEM image results also corroborate the CFU results, which showed that the fewest clean surfaces were found in the control, and this gradually increased with KKL and MCAE. SARS-CoV-2 inhibition, indicated by its TCID50 value, demonstrated that KKL almost completely inhibited SARS-CoV-2 infection and replication (99.99% reduction). Conclusion: KKL possesses antimicrobial efficacy on C. albicans biofilm on PMMA and SARS-CoV-2.
ISSN:2673-947X
2673-947X
DOI:10.3390/hygiene4010006