High-Throughput Screening of a Functional Human CXCL12-CXCR4 Signaling Axis in a Genetically Modified S. cerevisiae: Discovery of a Novel Up-Regulator of CXCR4 Activity

• Published in: Frontiers in molecular biosciences Vol. 7; p. 164
• Main Authors: Murphy, James W., Rajasekaran, Deepa, Merkel, Janie, Skeens, Erin, Keeler, Camille, Hodsdon, Michael E., Lisi, George P., Lolis, Elias
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 16.07.2020
• Subjects: chemokine; CXCL12; CXCR4; fosfosal; G protein-coupled receptor; high-throughput screening; Molecular Biosciences

CXCL12 activates CXCR4 and is involved in embryogenesis, hematopoiesis, and angiogenesis. It has pathological roles in HIV-1, WHIM disease, cancer, and autoimmune diseases. An antagonist, AMD3100, is used for the release of CD34+ hematopoietic stem cells from the bone marrow for autologous transplan...