Functionally acceptable substitutions in two alpha-helical regions of lambda repressor

• Published in: Proteins, structure, function, and bioinformatics Vol. 7; no. 4; p. 306
• Main Authors: Reidhaar-Olson, J F, Sauer, R T
• Format: Journal Article
• Language: English
• Published: United States 1990
• Subjects: Amino Acid Sequence; DNA-Binding Proteins; Escherichia coli - genetics; Membrane Proteins; Molecular Sequence Data; Monte Carlo Method; Mutation; Protein Conformation; Repressor Proteins - genetics; Transcription Factors - genetics; Viral Proteins; Viral Regulatory and Accessory Proteins

A method of targeted random mutagenesis has been used to investigate the informational content of 25 residue positions in two alpha-helical regions of the N-terminal domain of lambda repressor. Examination of the functionally allowed sequences indicates that there is a wide range in tolerance to ami...