Microcalorimetric Evaluation of the Effect of Kanamycin： An Analysis Based on the Median-Effect Principle

• Published in: Chinese journal of chemistry Vol. 25; no. 1; pp. 20 - 24
• Main Author: 王莉衡 范代娣 尚龙安 施惠娟 马晓轩 米钰 顾利锋 徐抗震
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 2007; WILEY‐VCH Verlag
• Subjects: kanamycin; microcalorimetry; recombinant Escherichia coli; 中效原理; 剂量效应; 卡那霉素; 微量热法; 药效评价; 重组大肠埃希氏菌
• ISSN: 1001-604X; 1614-7065
• DOI: 10.1002/cjoc.200790011

A study of the effect of drug, kanamycin, on the growth metabolism of recombinant Escherichia coli B 1 was carded out by microcalorimeter monitoring of the metabolic activity of treated cells. Power-time curves of growing recombinant Escherichia coli cell suspensions, treated with different kanamycin doses, were recorded. The extent of the effect was evaluated by changes in the slopes of the microcalorimetric curves and the kinetics of the drug action was interpreted from the time at which these changes reached their maximum values and maintained their maximum values. Experimental dose-effect relationships conform to the median-effect principle of the mass-action law： fa/（1-fa）=（D/D50）^m. A plot of y=lg[（fa）^1-1]^-1 versus x=lg D gives the slope m, D50 and R∞. The experimental results revealed that high concentration of kanamycin had an inhibitory effect on the growth of recombinant Escherichia coli B 1 in the lg phase, and had a promoting effect in the stationary period. Moreover, it was demonstrated that microcalorimetry was a reliable method for the detection of modulatory effects in biology.