Bacillus subtilis PcrA Couples DNA Replication, Transcription, Recombination and Segregation

• Published in: Frontiers in molecular biosciences Vol. 7; p. 140
• Main Authors: Moreno-del Alamo, María, Torres, Rubén, Manfredi, Candela, Ruiz-Masó, José A., del Solar, Gloria, Alonso, Juan Carlos
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 21.07.2020
• Subjects: Molecular Biosciences; RecL16; Rep; replication fork stalling; replication-transcription conflict; RNA polymerase backtracking; UvrD

Bacillus subtilis PcrA abrogates replication-transcription conflicts in vivo and disrupts RecA nucleoprotein filaments in vitro. Inactivation of pcrA is lethal. We show that PcrA depletion lethality is suppressed by recJ (involved in end resection), recA (the recombinase), or mfd (transcription-coup...