Two isoforms of aquaporin 2 responsive to hypertonic stress in bottlenose dolphin

• Published in: Journal of experimental biology Vol. 219; no. Pt 8; p. 1249
• Main Authors: Suzuki, Miwa, Wakui, Hitomi, Itou, Takuya, Segawa, Takao, Inoshima, Yasuo, Maeda, Ken, Kikuchi, Kiyoshi
• Format: Journal Article
• Language: English
• Published: England 15.04.2016
• Subjects: Amino Acid Sequence; Animals; Aquaporin 2 - chemistry; Aquaporin 2 - genetics; Aquaporin 2 - metabolism; Bottle-Nosed Dolphin - physiology; Cell Death - drug effects; Cell Membrane Permeability - drug effects; Cells, Cultured; Conserved Sequence - genetics; DNA, Complementary - genetics; DNA, Complementary - isolation & purification; Gene Expression Profiling; Gene Knockdown Techniques; Hypertonic Solutions - pharmacology; Introns - genetics; Kidney - drug effects; Kidney - metabolism; Mannitol - pharmacology; Oocytes - drug effects; Oocytes - metabolism; Open Reading Frames - genetics; Protein Isoforms - chemistry; Protein Isoforms - genetics; Protein Isoforms - metabolism; RNA Interference - drug effects; RNA Splicing - drug effects; RNA Splicing - genetics; RNA, Small Interfering - metabolism; Sodium Chloride - pharmacology; Software; Stress, Physiological - drug effects; Transcription, Genetic - drug effects; Water; Alternative splicing; Cellular osmoregulation; Osmotic tolerance; Cetacea

This study investigated the expression of aquaporin 2 (AQP2) and its newly found alternatively spliced isoform (alternative AQP2) and the functions of these AQP2 isoforms in the cellular hyperosmotic tolerance in the bottlenose dolphin Tursiops truncatus. mRNA sequencing revealed that alternative AQ...