Lichen secondary metabolites as DNA-interacting agents
•Interactions of selected lichen secondary metabolites 1–4 with calf thymus DNA were studied.•Binding constants of 4.3×105–2.4×107M−1 were determined from titration.•Among all studied compounds, only gyrophoric acid was shown to inhibit Topo I. A series of lichen secondary metabolites (parietin, atr...
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Published in | Toxicology in vitro Vol. 28; no. 2; pp. 182 - 186 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
01.03.2014
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Subjects | |
Online Access | Get full text |
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Summary: | •Interactions of selected lichen secondary metabolites 1–4 with calf thymus DNA were studied.•Binding constants of 4.3×105–2.4×107M−1 were determined from titration.•Among all studied compounds, only gyrophoric acid was shown to inhibit Topo I.
A series of lichen secondary metabolites (parietin, atranorin, usnic and gyrophoric acid) and their interactions with calf thymus DNA were investigated using molecular biophysics and biochemical methods. The binding constants K were estimated to range from 4.3×105 to 2.4×107M−1 and the percentage of hypochromism was found to be 16–34% (from spectral titration). The results of spectral measurement indicate that the compounds act as effective DNA-interacting agents. Electrophoretic separation studies prove that from all the metabolites tested in this study, only gyrophoric acid exhibited an inhibitory effect on Topo I (25μM). |
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ISSN: | 0887-2333 1879-3177 |
DOI: | 10.1016/j.tiv.2013.11.003 |