Complete shift of ferritin oligomerization toward nanocage assembly via engineered protein-protein interactions

• Published in: Chemical communications (Cambridge, England) Vol. 49; no. 34; pp. 3528 - 3530
• Main Authors: Ardejani, Maziar S, Chok, Xiao Ling, Foo, Ce Jin, Orner, Brendan P
• Format: Journal Article
• Language: English
• Published: England 01.01.2013
• Subjects: Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Cytochrome b Group - chemistry; Cytochrome b Group - genetics; Ferritins - chemistry; Ferritins - genetics; Mutation; Nanostructures - chemistry; Protein Conformation; Protein Engineering

Computational redesign of a dimorphic protein nano-cage at the C3-symmetrical interfaces forces it to assemble into the monomorphic cage. These monodisperse assemblies are at least 20 °C more stable than the parent. This approach adds to the toolkit of bottom-up molecular design with applications in...