Quantitative profile of lipid classes in blood by normal phase chromatography with evaporative light scattering detector: Application in the detection of lipid class abnormalities in liver cirrhosis

• Published in: Clinica chimica acta Vol. 421; pp. 132 - 139
• Main Authors: Chamorro, Laura, García-Cano, Ana, Busto, Rebeca, Martínez-González, Javier, Albillos, Agustín, Lasunción, Miguel Ángel, Pastor, Óscar
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 05.06.2013
• Subjects: Calibration; Chromatography; Chromatography, High Pressure Liquid - methods; Erythrocytes - chemistry; Fatty Acids, Nonesterified - blood; Fatty Acids, Nonesterified - classification; HPLC; Humans; Light; Lipid classes; Lipid metabolism; Lipidomics; Liver - metabolism; Liver - pathology; Liver cirrhosis; Liver Cirrhosis - blood; Liver Cirrhosis - pathology; Phospholipids - blood; Phospholipids - classification; Scattering, Radiation; Sensitivity and Specificity; Triglycerides - blood; Triglycerides - classification; FFA; PS; SPM; THF; ELSD; MG; Lipid metabolism; NPLC; HILIC; HPLC; LOD; CE; Rt; MS; Lipid classes; ESI-MS; CL; Lipidomics; Chromatography; LOQ; CT; LC-MS; TG; PC; CV; MELD; PE; PG; ISTD; PI; PL; FC; Liver cirrhosis

The lack of analytical methods specific for each lipid class, particularly for phospholipids and sphyngolipids, makes necessary their separation by preparative techniques before quantification. LC–MS would be the election method but for daily work in the clinical laboratory this is not feasible for...