Somatic Cancer Mutations in the SUV420H1 Protein Lysine Methyltransferase Modulate Its Catalytic Activity

• Published in: Journal of molecular biology Vol. 431; no. 17; pp. 3068 - 3080
• Main Authors: Bröhm, Alexander, Elsawy, Hany, Rathert, Philipp, Kudithipudi, Srikanth, Schoch, Tabea, Schuhmacher, Maren Kirstin, Weirich, Sara, Jeltsch, Albert
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 09.08.2019
• Subjects: H4K20 methylation; histone methylation; protein lysine methylation; protein lysine methyltransferase; somatic cancer mutations; histone methylation; CD; BRD1; H4K20 methylation; PKMT; dko; GR; protein lysine methylation; protein lysine methyltransferase; somatic cancer mutations; AdoMet; MEF; Somatic cancer mutations; Protein lysine methylation; Protein lysine methyltransferase; Histone methylation

SUV420H1 is a protein lysine methyltransferase that introduces di- and trimethylation of H4K20 and is frequently mutated in human cancers. We investigated the functional effects of eight somatic cancer mutations on SUV420H1 activity in vitro and in cells. One group of mutations (S255F, K258E, A269V)...