Cooperativity between an Upstream TATA-like Sequence and a CAA Repeated Element Mediates E1A-dependent Negative Repression of the H-2K▪ Class I Gene (∗)

• Published in: The Journal of biological chemistry Vol. 270; no. 5; pp. 2327 - 2336
• Main Authors: Tang, Xiaoren, Li, Hai-Ou, Sakatsume, Osamu, Ohta, Tomohiro, Tsutsui, Hatsumi, Smit, Arian F.A., Horikoshi, Masami, Kourilsky, Phillipe, Israël, Alain, Gachelin, Gabriel, Yokoyama, Kazushige
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 03.02.1995; American Society for Biochemistry and Molecular Biology
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.270.5.2327

In primary rodent cells transformed by the E1A region of the highly oncogenic adenovirus type 12, repression of transcription mediated by the far upstream TATA-like element was observed only in conjunction with either possible juxtaposition of a CAA repeated element in the presence of E1A and was dependent upon the relative arrangement of both the TATA-like and CAA repeated motifs in both homologous and heterologous promoter constructs. A gel shift competition study demonstrated that the TATA-binding protein (TBP) or a TBP-like protein can bind to both the upstream TATA-like sequence and the regular TATA box on the H-2K▪ basal promoter. Moreover, employing immunoselection and cyclic amplification and selection of targets (CASTing) methods with nuclear extracts derived from Ad12-E1A transformants, we have identified a high affinity binding site in the H-2K▪ class I promoter for E1A-associated DNA-binding proteins. The sequences of the binding sites were identified and were found to contain both the upstream TATA-like motif and the CAA repeated motifs. Our results suggest that the TATA-like sequence in the far upstream region of the H-2K▪ gene is one of the elements that is required for Ad12-E1A-mediated negative repression.