Three-dimensional structure analysis of melanocytes and keratinocytes in senile lentigo
Senile lentigo or age spots are hyperpigmented macules of skin that commonly develop following long-term exposure to ultraviolet radiation. This condition is caused by accumulation of large numbers of melanosomes (melanin granules) produced by melanocytes within neighboring keratinocytes. However, t...
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Published in | Microscopy Vol. 70; no. 2; p. 224 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
01.04.2021
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Abstract | Senile lentigo or age spots are hyperpigmented macules of skin that commonly develop following long-term exposure to ultraviolet radiation. This condition is caused by accumulation of large numbers of melanosomes (melanin granules) produced by melanocytes within neighboring keratinocytes. However, there is still no consensus regarding the melanosome transfer mechanism in senile lentigo. To date, most pathohistological studies of skin have been two-dimensional and do not provide detailed data on the complex interactions of the melanocyte-keratinocyte network involved in melanosome transfer. We performed a three-dimensional reconstruction of the epidermal microstructure in senile lentigo using three different microscopic modalities to visualize the topological melanocyte-keratinocyte relationship and melanosome distribution. Confocal laser microscopy images showed that melanocyte dendritic processes are more frequently branched and elongated in senile lentigo skin than in normal skin. Serial transmission electron micrographs showed that dendritic processes extend into intercellular spaces between keratinocytes. Focused ion beam-scanning electron micrographs showed that dendritic processes in senile lentigo encircle adjacent keratinocytes and accumulate large numbers of melanosomes. Moreover, melanosomes transferred to keratinocytes are present not only in the supranuclear area but throughout the perinuclear area except on the basal side. The use of these different microscopic methods helped to elucidate the three-dimensional morphology and topology of melanocytes and keratinocytes in senile lentigo. We show that the localization of melanosomes in dendritic processes to the region encircling recipient keratinocytes contributes to efficient melanosome transfer in senile lentigo. |
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AbstractList | Senile lentigo or age spots are hyperpigmented macules of skin that commonly develop following long-term exposure to ultraviolet radiation. This condition is caused by accumulation of large numbers of melanosomes (melanin granules) produced by melanocytes within neighboring keratinocytes. However, there is still no consensus regarding the melanosome transfer mechanism in senile lentigo. To date, most pathohistological studies of skin have been two-dimensional and do not provide detailed data on the complex interactions of the melanocyte-keratinocyte network involved in melanosome transfer. We performed a three-dimensional reconstruction of the epidermal microstructure in senile lentigo using three different microscopic modalities to visualize the topological melanocyte-keratinocyte relationship and melanosome distribution. Confocal laser microscopy images showed that melanocyte dendritic processes are more frequently branched and elongated in senile lentigo skin than in normal skin. Serial transmission electron micrographs showed that dendritic processes extend into intercellular spaces between keratinocytes. Focused ion beam-scanning electron micrographs showed that dendritic processes in senile lentigo encircle adjacent keratinocytes and accumulate large numbers of melanosomes. Moreover, melanosomes transferred to keratinocytes are present not only in the supranuclear area but throughout the perinuclear area except on the basal side. The use of these different microscopic methods helped to elucidate the three-dimensional morphology and topology of melanocytes and keratinocytes in senile lentigo. We show that the localization of melanosomes in dendritic processes to the region encircling recipient keratinocytes contributes to efficient melanosome transfer in senile lentigo. |
Author | Ohta, Keisuke Mizutani, Yuki Hayashi, Akinobu Yamashita, Mika Hashimoto, Rie Rikimaru-Nishi, Yukiko Atsugi, Toru Ryu, Akemi |
Author_xml | – sequence: 1 givenname: Yuki surname: Mizutani fullname: Mizutani, Yuki organization: Research Laboratories, KOSÉ Corporation, 48-18 Sakae-cho, Kita-ku, Tokyo, 114-0005 Japan – sequence: 2 givenname: Mika surname: Yamashita fullname: Yamashita, Mika organization: Research Laboratories, KOSÉ Corporation, 48-18 Sakae-cho, Kita-ku, Tokyo, 114-0005 Japan – sequence: 3 givenname: Rie surname: Hashimoto fullname: Hashimoto, Rie organization: Research Laboratories, KOSÉ Corporation, 48-18 Sakae-cho, Kita-ku, Tokyo, 114-0005 Japan – sequence: 4 givenname: Toru surname: Atsugi fullname: Atsugi, Toru organization: Research Laboratories, KOSÉ Corporation, 48-18 Sakae-cho, Kita-ku, Tokyo, 114-0005 Japan – sequence: 5 givenname: Akemi surname: Ryu fullname: Ryu, Akemi organization: Research Laboratories, KOSÉ Corporation, 48-18 Sakae-cho, Kita-ku, Tokyo, 114-0005 Japan – sequence: 6 givenname: Akinobu surname: Hayashi fullname: Hayashi, Akinobu organization: Research Laboratories, KOSÉ Corporation, 48-18 Sakae-cho, Kita-ku, Tokyo, 114-0005 Japan – sequence: 7 givenname: Yukiko surname: Rikimaru-Nishi fullname: Rikimaru-Nishi, Yukiko organization: Department of Plastic and Reconstructive Surgery, and Maxillofacial Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan – sequence: 8 givenname: Keisuke surname: Ohta fullname: Ohta, Keisuke organization: Division of Microscopic and Developmental Anatomy, Department of Anatomy, Kurume University School of Medicine, Kurume, Fukuoka, 830-0011 Japan |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32991711$$D View this record in MEDLINE/PubMed |
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Copyright | The Author(s) 2020. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. |
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Keywords | FIB-SEM cell–cell interaction melanocyte melanosome senile lentigo three-dimensional structure |
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Title | Three-dimensional structure analysis of melanocytes and keratinocytes in senile lentigo |
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