Gold nanoparticles based sensor for in vitro analysis of drug-drug interactions using imipramine and isoniazid drugs: A proof of concept approach

• Published in: Sensors and actuators. B, Chemical Vol. 252; pp. 1055 - 1062
• Main Authors: Shaikh, Tayyaba, Pandey, Ashish, Sirajuddin, Talpur, Farah N., Kaushik, Aman, Niazi, Javed H.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.11.2017; Elsevier Science Ltd
• Subjects: Antibiotics; Antidepressants; Computer simulation; Drug dosages; Drug-drug interaction; Drugs; Gold; Gold nanoparticles; Imipramine; Isoniazid; Molecular docking; Nanoparticles; Organic chemistry; Predictions; Tuberculosis; Imipramine; Drug-drug interaction; Isoniazid; Gold nanoparticles; Molecular docking
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2017.06.083

[Display omitted] •Drug conjugated gold nanoparticles (AuNPs) as drug-drug interaction (DDIs) sensor.•Computational simulations to interrogate DDIs between imipramine and isoniazid.•DDIs were induced by their carrier charges electrostatically.•Interaction between the two drugs was validated by electrochemical measurements. Prediction of drug-drug interactions (DDIs) before their manifestation is of paramount importance in the development of drug-dosing protocols and drug-design. In this context, we proposed for the first time, gold nanopaticles (AuNPs) based sensor for in vitro assessment of DDIs. Here, we first conducted in-situ molecular docking and simulations studies to interrogate DDIs that allowed predictions of DDIs between two model drugs, such as imipramine (antidepressant) and isoniazid (anti-tuberculosis). This was followed by in vitro studies involving imipramine conjugated gold nanoparticles (Imipramine-AuNPs) as probes for screening DDIs. Association of isoniazid with imipramine molecules over the surface of AuNPs sensor occurred that was confirmed by a combination of physico-chemical methods. Electrochemical investigations revealed the quantitative association of imipramine and isoniazid molecules, where a linear relationship of peak current (Ip) was observed for increasing concentrations of isoniazid from 31 to 667μM at Imipramine-AuNPs modified Screen Printed Electrodes (SPE), validating the predicted DDIs. The current study aims to highlight the possible interaction between the two drugs that provides valuable information useful for the prediction of similar types of DDIs with other drug pairs.