Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu2+ in Cells
Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity...
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| Published in | Molecules (Basel, Switzerland) Vol. 29; no. 5; p. 1020 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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| Abstract | Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu2+. With the combination of Cu2+, the probe was subjected to fluorescence quenching. The research suggested that the probe FP carried out the highly sensitive detection of Cu2+ with detection limits of 1.7 nM. The fluorescence quenching of fluorescamine was induced by Cu2+ perhaps due to the PET (photoinduced electron transfer) mechanism. The FP-Cu2+ complex shows weak fluorescence, which is likely due to the PET quenching effect from Cu2+ to fluorescamine fluorophore. Moreover, the probe FP can be employed for imaging Cu2+ in living cells. The new fluorescent probe developed in this study shows the advantages of good biocompatibility and low cytotoxicity. It can be adopted for the targeted detection of Cu2+ in cells, and it has promising applications in the mechanism research and diagnosis of Cu2+-associated diseases. |
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| AbstractList | Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu2+. With the combination of Cu2+, the probe was subjected to fluorescence quenching. The research suggested that the probe FP carried out the highly sensitive detection of Cu2+ with detection limits of 1.7 nM. The fluorescence quenching of fluorescamine was induced by Cu2+ perhaps due to the PET (photoinduced electron transfer) mechanism. The FP-Cu2+ complex shows weak fluorescence, which is likely due to the PET quenching effect from Cu2+ to fluorescamine fluorophore. Moreover, the probe FP can be employed for imaging Cu2+ in living cells. The new fluorescent probe developed in this study shows the advantages of good biocompatibility and low cytotoxicity. It can be adopted for the targeted detection of Cu2+ in cells, and it has promising applications in the mechanism research and diagnosis of Cu2+-associated diseases. Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu 2+ . With the combination of Cu 2+ , the probe was subjected to fluorescence quenching. The research suggested that the probe FP carried out the highly sensitive detection of Cu 2+ with detection limits of 1.7 nM. The fluorescence quenching of fluorescamine was induced by Cu 2+ perhaps due to the PET (photoinduced electron transfer) mechanism. The FP-Cu 2+ complex shows weak fluorescence, which is likely due to the PET quenching effect from Cu 2+ to fluorescamine fluorophore. Moreover, the probe FP can be employed for imaging Cu 2+ in living cells. The new fluorescent probe developed in this study shows the advantages of good biocompatibility and low cytotoxicity. It can be adopted for the targeted detection of Cu 2+ in cells, and it has promising applications in the mechanism research and diagnosis of Cu 2+ -associated diseases. |
| Author | Chen, Yi Qin, Ziying Song, Zhen Li, Lin Ren, Jing Han, Hongfei |
| AuthorAffiliation | 2 Department of Chemistry, Taiyuan Normal University, Jinzhong 030619, China 1 Laboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, China |
| AuthorAffiliation_xml | – name: 2 Department of Chemistry, Taiyuan Normal University, Jinzhong 030619, China – name: 1 Laboratory of Protein Based Functional Materials of Shanxi Province, Taiyuan Normal University, Jinzhong 030619, China |
| Author_xml | – sequence: 1 givenname: Jing surname: Ren fullname: Ren, Jing – sequence: 2 givenname: Lin surname: Li fullname: Li, Lin – sequence: 3 givenname: Hongfei surname: Han fullname: Han, Hongfei – sequence: 4 givenname: Yi surname: Chen fullname: Chen, Yi – sequence: 5 givenname: Ziying surname: Qin fullname: Qin, Ziying – sequence: 6 givenname: Zhen surname: Song fullname: Song, Zhen |
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| SubjectTerms | Amino acids Binding sites Biocompatibility Copper copper(II) ion E coli Enzymes probe protein Proteins Scientific imaging |
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| Title | Construction of a New Probe Based on Copper Chaperone Protein for Detecting Cu2+ in Cells |
| URI | https://www.proquest.com/docview/2955897232 https://search.proquest.com/docview/2956687004 https://pubmed.ncbi.nlm.nih.gov/PMC10935257 https://doaj.org/article/dbca358cc54942ff8fcf7e2360d575fa |
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