One-step molecular detection of the MYD88 L265P mutation by unlabeled probe genotyping analysis

• Published in: Molecular and cellular probes Vol. 29; no. 1; pp. 74 - 77
• Main Authors: Poon, Kok-Siong, Liu, Te-Chih, Tan, Karen Mei-Ling, Koay, Evelyn Siew-Chuan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2015
• Subjects: Antigens, Differentiation - genetics; Genotype; High-resolution melt; Humans; L265P; Leucine - genetics; Molecular Diagnostic Techniques - economics; Molecular Diagnostic Techniques - methods; Mutation; MYD88; Oligonucleotide Probes - genetics; Proline - genetics; Reproducibility of Results; Sequence Analysis, DNA; Unlabeled probe; Waldenstrom Macroglobulinemia - cerebrospinal fluid; Waldenstrom Macroglobulinemia - diagnosis; Waldenstrom Macroglobulinemia - genetics; Waldenstrӧm macroglobulinemia; Unlabeled probe; Waldenstrӧm macroglobulinemia; L265P; High-resolution melt; MYD88

The aim of our study was to establish an unlabeled probe genotyping approach for rapid detection of the MYD88 L265P mutation in the differential diagnosis of Waldenstrӧm macroglobulinemia patients. Analytical and clinical validation of the assay was performed using serially diluted amplicon-cloned s...