Epigenetic Regulation of the ERβ Gene on the Estrogen Signal Transfection Pathway in Colon Cancer Cells

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 30; no. 1; pp. 69 - 74
• Main Author: 翟荣林 王国斌 蔡开琳 陶凯雄 许飞 张万里 王智勇
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01.02.2010; Department of General Surgery,Union Hospital,Tong]i Medical College,Huazhong University of Science and Technology,Wuhan430022,China
• Subjects: Medicine; Medicine & Public Health; signal transfection; methylation; colon cancer; epigenetics; estrogen receptor β
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-010-0112-6

We studied the regulatory effects of the estragen receptorβ(ERβ)gene on the downstream estrogen signal transfection pathway in colon cancer cells and the possible mechanisms involved.A human ERβ gene recombinant expression plasmid,pEGFP-C1-ERβ,was constructed and transfected into the Caco-2 colon cancer cell line,a line with low ERβ gene expression.The expression of ERβ mRNA and protein was detected 72h after transfection.RT-PCR was used to examine the expression levels of the progesterone recepror(PR)gene ...