Phenotype- and species-specific skin proteomic signatures for incision-induced pain in humans and mice

• Published in: British journal of anaesthesia : BJA Vol. 130; no. 3; pp. 331 - 342
• Main Authors: Segelcke, Daniel, van der Burgt, Max, Kappert, Christin, Schmidt Garcia, Daniela, Sondermann, Julia R., Bigalke, Stephan, Pradier, Bruno, Gomez-Varela, David, Zahn, Peter K., Schmidt, Manuela, Pogatzki-Zahn, Esther M.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2023
• Subjects: acute pain; Animals; chronic pain; chronic post-surgical pain; Humans; Hyperalgesia - prevention & control; incision; Male; Mice; Pain, Postoperative; phenotype; protein-protein interaction; Proteome; Proteomics; Skin - metabolism; surgical pain; translational; phenotype; chronic pain; proteomics; translational; acute pain; chronic post-surgical pain; incision; protein-protein interaction; surgical pain
• ISSN: 0007-0912; 1471-6771; 1471-6771
• DOI: 10.1016/j.bja.2022.10.040

Acute pain after surgery is common and often leads to chronic post-surgical pain, but neither treatment nor prevention is currently sufficient. We hypothesised that specific protein networks (protein-protein interactions) are relevant for pain after surgery in humans and mice. Standardised surgical incisions were performed in male human volunteers and male mice. Quantitative and qualitative sensory phenotyping were combined with unbiased quantitative mass spectrometry-based proteomics and protein network theory. The primary outcomes were skin protein signature changes in humans and phenotype-specific protein-protein interaction analysis 24 h after incision. Secondary outcomes were interspecies comparison of protein regulation as well as protein-protein interactions after incision and validation of selected proteins in human skin by immunofluorescence. Skin biopsies in 21 human volunteers revealed 119/1569 regulated proteins 24 h after incision. Protein-protein interaction analysis delineated remarkable differences between subjects with small (low responders, n=12) and large incision-related hyperalgesic areas (high responders, n=7), a phenotype most predictive of developing chronic post-surgical pain. Whereas low responders predominantly showed an anti-inflammatory protein signature, high responders exhibited signatures associated with a distinct proteolytic environment and persistent inflammation. Compared to humans, skin biopsies in mice habored even more regulated proteins (435/1871) 24 h after incision with limited overlap between species as assessed by proteome dynamics and PPI. Immunohistochemistry confirmed the expression of high priority candidates in human skin biopsies. Proteome profiling of human skin after incision revealed protein-protein interactions correlated with pain and hyperalgesia, which may be of potential significance for preventing chronic post-surgical pain. Importantly, protein-protein interactions were differentially modulated in mice compared to humans opening new avenues for successful translational research.