Potential role of MicroRNA 200c gene expression in assessment of colorectal cancer

• Published in: Archives of biochemistry and biophysics Vol. 647; pp. 41 - 46
• Main Authors: Tayel, Safaa I., Fouda, Eman A.M., Gohar, Suzy F., Elshayeb, Elsayed I., El-sayed, Eman H., El-kousy, Salah M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2018
• Subjects: Adult; Aged; Biomarkers, Tumor - genetics; CA19-9; Carcinoembryonic antigen; Colorectal cancer; Colorectal Neoplasms - diagnosis; Colorectal Neoplasms - genetics; Female; Gene Expression Regulation, Neoplastic; Humans; Kaplan-Meier Estimate; Male; MicroRNA200c; MicroRNAs - genetics; Middle Aged; Prognosis; Real time PCR; Up-Regulation; Carcinoembryonic antigen; MicroRNA200c; Real time PCR; CA19-9; Colorectal cancer
• DOI: 10.1016/j.abb.2018.04.009
• ISSN: 0003-9861

Colorectal cancer (CRC) is a common cancer worldwide that affects men and women of all racial and ethnic groups. Recent evidence supports the role of microRNAs in CRC. We planned to investigate microRNA200c expression and its relation with diagnosis, prognosis, metastasis and overall survival in CRC patients. This study enrolled 90 subjects (3’0 CRC patients, 30 patients with benign colorectal polyps and 30 healthy control subjects). Laboratory investigations included measurement of serum CA19-9 and CEA by enzyme linked immunosorbent assay (ELISA) method and relative quantitation (RQ) of microRNA200c gene expression by real time PCR technique. Significant higher MicroRNA200c expression levels in CRC patients versus both benign (P < 0.011) and control groups (P < 0.001), additionally, benign group had elevated levels versus control (P < 0.001). MicroRNA 200c at cutoff >4.56 had sensitivity 86.67% and specificity 73.33% (P < 0.001) for CRC discrimination. Kaplan-Meier survival analysis revealed significant association (P = 0.028) of high expression of microRNA200c with decreased overall survival. Noticeable up-regulation of microRNA200c in CRC and its remarkable relation with unfavorable survival suggesting its potential dual use as a diagnostic and prognostic biomarker for CRC.