Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models
non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discove...
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Published in | Revista española de enfermedades digestivas Vol. 111; no. 4; pp. 301 - 307 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
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Spain
01.01.2019
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Abstract | non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management.
this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated.
HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation.
in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing. |
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AbstractList | non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management.
this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated.
HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation.
in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing. INTRODUCTIONnon-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management. MATERIAL AND METHODSthis study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated. RESULTSHF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation. CONCLUSIONin conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing. |
Author | Rojas, Ángela Gallego-Durán, Rocío Gil-Gómez, Antonio Cádernas-García, Antonio Sánchez Torrijos, Yolanda Muñoz-Hernández, Rocío Ampuero, Javier Montero-Vallejo, Rocío Gato, Sheila Romero-Gómez, Manuel Álvarez-Amor, Leticia Martín, Francisco Maya-Miles, Douglas |
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Snippet | non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy... INTRODUCTIONnon-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved... |
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SubjectTerms | Alanine Transaminase - blood Animals Aspartate Aminotransferases - blood Body Weight Cholesterol - blood Choline Diet, High-Fat Disease Models, Animal Fructose - administration & dosage Liver - enzymology Liver - pathology Male Methionine - administration & dosage Mice Mice, Inbred C57BL Non-alcoholic Fatty Liver Disease - blood Non-alcoholic Fatty Liver Disease - etiology Non-alcoholic Fatty Liver Disease - pathology Random Allocation Sweetening Agents - administration & dosage |
Title | Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models |
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