Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models

• Published in: Revista española de enfermedades digestivas Vol. 111; no. 4; pp. 301 - 307
• Main Authors: Gallego-Durán, Rocío, Álvarez-Amor, Leticia, Gil-Gómez, Antonio, Rojas, Ángela, Muñoz-Hernández, Rocío, Cádernas-García, Antonio, Maya-Miles, Douglas, Montero-Vallejo, Rocío, Gato, Sheila, Sánchez Torrijos, Yolanda, Ampuero, Javier, Martín, Francisco, Romero-Gómez, Manuel
• Format: Journal Article
• Language: English
• Published: Spain 01.01.2019
• Subjects: Alanine Transaminase - blood; Animals; Aspartate Aminotransferases - blood; Body Weight; Cholesterol - blood; Choline; Diet, High-Fat; Disease Models, Animal; Fructose - administration & dosage; Liver - enzymology; Liver - pathology; Male; Methionine - administration & dosage; Mice; Mice, Inbred C57BL; Non-alcoholic Fatty Liver Disease - blood; Non-alcoholic Fatty Liver Disease - etiology; Non-alcoholic Fatty Liver Disease - pathology; Random Allocation; Sweetening Agents - administration & dosage
• ISSN: 1130-0108
• DOI: 10.17235/reed.2019.6083/2018

non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management. this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated. HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation. in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testing.